larval dendritic arborizing neurons display loss of the multipolar layout of dendrites, with many becoming bipolar.
larval es neurons not do not show a significant change in morphology as compared to wild type.
mutants, major dendrites of class IV da neurons are either missing or shortened, consequently resulting in a reduced dendritic arbor. In contrast, the axonal growth of these neurons is normal. Dendrites of class I da neurons are also severely shortened.
mutants, generated by crossing dar13232
germline clones with dar13010
mutants show the same phenotype as dar13010
zygotic mutants, that is, the dendrites are reduced but the axons are normal. Dendritic defects are visible as early as 16-17 hours after egg laying and become more pronounced in 18-19 hours after egg laying, suggesting these mutants exhibit reduced outgrowth of dendrites.
Single neurons homozygous for dar13010
, generated through MARCM, exhibit severely reduced dendritic arbors, supporting the notion that dar1
functions cell-autonomously. Dendrites are dramatically reduced in all four classes of da neurons (class I-ddaD and ddaE, class II-ddaB, class III-ddaA, and class IV-ddaC). In contrast, there is no significant defect in axonal growth in these mutants.