A wild-type Hsap\UBB-monomer is expressed under the control of tetO operator sequences.
increased cell death | P-stage, with Tn10\tetRrtTA.Act5C
lethal - all die before end of P-stage, with Tn10\tetRrtTA.Act5C
long lived | male limited, with Scer\GAL4da.G32, Tn10\tetRrtTA(M2-alt).UAS
melanotic mass phenotype | P-stage, with Tn10\tetRrtTA.Act5C
some die during P-stage, with Tn10\tetRrtTA.Act5C
pupa, with Tn10\tetRrtTA.Act5C
Overexpression of Hsap\UBBTn10\tetO.cHa under the control of Tn10\tetRAct5C.T:Hsim\VP16 results in lethality. The lethality caused by Hsap\UBBTn10\tetO.cHa-overexpression is associated with a dramatic disruption of normal pupal structures and large melanotic inclusions indicative of extensive cell death.
Overexpression of Hsap\UBBTn10\tetO.cHa under the control of Scer\GAL4da.G32 and Tn10\tetRM2.alt.Scer\UAS.T:Hsim\VP16 does not decrease the survival-rate in adults. Instead, overexpression of Hsap\UBB+1.Tn10\tetO results in a slight increase in male life span.