UASp regulatory sequences drive expression of an artificial microRNA (shRNA) targeting tud.
grandchildless, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG
Expression of tuddsRNA.shRNA.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 and Scer\GAL4nos.PG results in a grandchild-less phenotype.