FB2025_01 , released February 20, 2025
Allele: Dmel\MCUKK108726
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General Information
Symbol
Dmel\MCUKK108726
Species
D. melanogaster
Name
FlyBase ID
FBal0259720
Feature type
allele
Associated gene
Dmel\MCU
Associated Insertion(s)
Carried in Construct
P{KK108726}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK108726}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK108726
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of MCUKK108726 driven by Scer\GAL4Act.PU or Scer\GAL4tub.PU results in lethality before adulthood.

      Expression of MCUKK108726 (together with UAS-Dicer2, to enhance RNAi efficiency) driven by Scer\GAL4nSyb.PS or Scer\GAL4238Y, but not when driven by Scer\GAL4VT064246, Scer\GAL4ple.PF, Scer\GAL4GH146, Scer\GAL4Mz604, Scer\GAL4NP2492, Scer\GAL4NP2631, Scer\GAL4G0431, Scer\GAL4Orco.2.642.Hsim\VP22, Scer\GAL4c305a, Scer\GAL4GMR35B12, Scer\GAL4c739, Scer\GAL4GMR28H05, Scer\GAL4GMR16A06, or Scer\GAL4GMR11D09, impairs adult olfactory intermediate-term memory in olfactory aversive tests, as compared to controls.

      Expression of MCUKK108726, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 or Scer\GAL4Tab2-201Y impairs intermediate-term memory in olfactory aversive tests (but not olfactory avoidance, shock avoidance, learning, or immediate or long-term memory) as compared to controls; the impairment persists after either anesthesia-sensitive memory elimination by cold-shock treatment or 5-times massed training.

      Expression of MCUKK108726, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 or Scer\GAL4Tab2-201Y and temporally-limited by Gal80[ts] to pupation or pre-adulthood developing stages by Gal80[ts], respectively, impairs adult olfactory intermediate-term memory in olfactory aversive tests as compared to controls.

      Expression of MCUKK108726, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR13F02 results in axons of adult mushroom body α and β neurons being longer and bigger, but with similar number of nodes, as compared to controls; mushroom body γ neurons appear unaltered.

      Expression of MCUKK108726, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR16A06 does not impair adult intermediate-term olfactory memory.

      Expression of MCUKK108726, with UAS-Dicer2 to enhance RNAi efficiency, driven by Scer\GAL4GMR25H11 impairs adult olfactory intermediate-term memory in olfactory aversive tests (but not odor avoidance or shock avoidance) as compared to controls.

      (Drago and Davis, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG18769KK108726
      MCUKK108726
      Name Synonyms
      Secondary FlyBase IDs
        References (4)