FlyBase Allele Report: Dmel\DIP-γ[MI03222]

General Information

Symbol

Dmel\DIP-γ^MI03222

Species

D. melanogaster

Name

FlyBase ID

FBal0260559

Feature type

allele

Associated gene

Dmel\DIP-γ

Associated Insertion(s)

Mi{MIC}DIP-γ^MI03222

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{MIC}DIP-γ^MI03222

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Also carries

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:28,957,558..28,957,558 [-]

(Courgeon and Desplan, 2019, Menon et al., 2019, Carrillo et al., 2015, Bellen, 2011.5.31)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with Df(3R)Exel6210)

(Carrillo et al., 2015)

abnormal neurophysiology | third instar larval stage (with Df(3R)Exel6210)

(Carrillo et al., 2015)

decreased cell number | adult stage (with Df(3R)Exel6210)

(Carrillo et al., 2015)

lethal

(Ozkan et al., 2013)

viable (with Df(3R)Exel6210)

(Carrillo et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

DIP-γ^MI03222/Df(3R)Exel6210 third instar larvae have extra satellite (supernumerary) boutons at the neuromuscular junction (NMJ) compared to wild type and heterozygotes (measured at muscle 4). DIP-γ^MI03222/Df(3R)Exel6210 third instar larvae show significant increases in miniature excitatory postsynaptic potential (mEPSP) amplitude and frequency (but no difference in evoked responses) compared to controls.

Terminals of R7 photoreceptor cells of yellow ommatidia are bulb-shaped and regularly arranged in layer 6 of the medulla (M6) in DIP-γ^MI03222/+ adult flies, with less than 10% of terminals having nub-like overshoots that extend deeper into M6; terminals in DIP-γ^MI03222/Df(3R)Exel6210 adults have irregular shapes, are not lined up in a fixed position along the vertical axis, and show a significant increase (30%) of overshoots, even extending into the M7 layer. DIP-γ^MI03222/Df(3R)Exel6210 adults have large gaps in medulla layer M6; DIP-γ- and (Dm8-specific driver) Scer\GAL4^ort.C2-positive Dm8 neurons are significantly reduced in the optic lobe compared to controls.

(Carrillo et al., 2015)

Mutant embryos show no visible defects in the CNS axon ladder.

(Ozkan et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

DIP-γ^MI03222/Df(3R)Exel6210 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

NOT Enhancer of

Statement

Reference

DIP-γ^MI03222/Df(3R)Exel6210 is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

Other

Statement

Reference

DIP-γ^MI03222/DIP-gamma[+], dpr11^MI02231 has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Dad^j1E4/Dad[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

DIP-γ^MI03222/Df(3R)Exel6210 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

DIP-γ^MI03222/Df(3R)Exel6210 has NMJ bouton | third instar larval stage | increased number phenotype, non-enhanceable by dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

NOT Enhancer of

Statement

Reference

DIP-γ^MI03222/Df(3R)Exel6210 is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

DIP-γ^MI03222/Df(3R)Exel6210 is a non-enhancer of NMJ bouton | third instar larval stage | increased number phenotype of dpr11^MI02231/Df(3R)BSC193

(Carrillo et al., 2015)

Other

Statement

Reference

DIP-γ^MI03222/DIP-gamma[+], dpr11^MI02231 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222/DIP-gamma[+], dpr11^MI02231 has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Dad^j1E4/Dad[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Dad^j1E4/Dad[+] has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Scer\GAL4^elav.PU, tkv^{Q199D.UAS.Tag:HA} has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

dpr11^MI02231/+;DIP-γ^MI03222/+ transheterozygote third instar larvae have extra satellite (supernumerary) boutons at the neuromuscular junction (NMJ) compared to wild type and heterozygotes (measured at muscle 4) and dpr11^MI02231,Df(3R)Exel6210/Df(3R)BSC193,DIP-γ^MI03222 double mutants show similar increases (non-enhanceable) in NMJ bouton number as dpr11^MI02231/Df(3R)BSC193 or DIP-γ^MI03222/Df(3R)Exel6210 third instar larvae.

DIP-γ^MI03222/Dad^j1E4 transheterozygote third instar larvae have extra satellite boutons at the neuromuscular junction (NMJ) compared to wild type and heterozygotes.

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^elav.PU in a DIP-γ^MI03222/+ background leads to extra satellite boutons at the neuromuscular junction (NMJ) of third instar larvae.

(Carrillo et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DIP-γ^MI03222/Df(3R)Exel6210 is rescued by Scer\GAL4^elav.PU/DIP-γ^UAS.cCa

(Carrillo et al., 2015)

DIP-γ^MI03222/Df(3R)Exel6210 is rescued by DIP-γ^UAS.cCa/Scer\GAL4^{Mhc.Switch.PO}

(Carrillo et al., 2015)

Comments

Expression of DIP-γ^Scer\UAS.cCa driven by Scer\GAL4^elav.PU or Scer\GAL4^{Mhc.Switch.PO} (with RU486 feeding) reduces satellite bouton number at the NMJ of DIP-γ^MI03222/Df(3R)Exel6210 third instar larvae.

(Carrillo et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35928

y¹ w^*; Mi{MIC}DIP-γ^MI03222/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG14521 MiMIC

(Ozkan et al., 2013)

CG14521^MI03222

(Bellen, 2011.5.31)

DIP-γ^MI03222

(Praschberger et al., 2023, Menon et al., 2019)

DIP-γ<up>MiMIC MI03222 5'>EGFP</up>

(Carrillo et al., 2015)

DIP-γ^MiMIC

(Carrillo et al., 2015)

DIPγ^MI03222

(Courgeon and Desplan, 2019)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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