FlyBase Allele Report: Dmel\fh[RNAi.UAS.cLa]

General Information

Symbol

Dmel\fh^RNAi.UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0260622

Feature type

allele

Associated gene

Dmel\fh

Associated Insertion(s)

Carried in Construct

P{UAS-fh.RNAi.L}

Also Known As

UAS-fhIR, fhRNAi-2, UAS-fhRNAi-2

Key Links

Transgenic product class

RNAi_reagent

(Llorens et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Llorens et al., 2007)

Mutagen

in vitro construct

(Llorens et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-fh.RNAi.L}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of fh sequences which are separated from each other by a spacer fragment.

(Llorens et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Friedreich ataxia

CEA

(Navarro et al., 2015, Soriano et al., 2013, Navarro et al., 2010, Llorens et al., 2007)

model of Friedreich ataxia 1

CEA

(Soriano et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Friedreich ataxia

is ameliorated by GLaz^UAS.cWa

(Navarro et al., 2010)

is ameliorated by Fer1HCH^UAS.cMa

(Navarro et al., 2015)

is ameliorated by mfrn^GD336

(Navarro et al., 2015)

is ameliorated by mfrn^NIG.4963R

(Navarro et al., 2015)

is ameliorated by Fer3HCH^UAS.cMa

(Navarro et al., 2015)

is ameliorated by Fer2LCH^UAS.cMa

(Navarro et al., 2015)

is exacerbated by mfrn^UAS.cNa

(Navarro et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^repo.PU

(Navarro et al., 2010)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Act.PU

(Soriano et al., 2013)

abnormal locomotor behavior | adult stage, with Scer\GAL4^{neur-GAL4-A101}

(Soriano et al., 2013)

abnormal locomotor behavior | adult stage | conditional, with Scer\GAL4^Act.PU

(Llorens et al., 2007)

abnormal oxidative stress response | adult stage, with Scer\GAL4^repo.PU

(Navarro et al., 2010)

chemical sensitive, with Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

(Llorens et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^how-24B

(Llorens et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^Ugt36A1.PK

(Llorens et al., 2007)

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^Act.PU

(Llorens et al., 2007)

short lived, with Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

short lived, with Scer\GAL4^Act.PU

(Soriano et al., 2013, Llorens et al., 2007)

short lived, with Scer\GAL4^{neur-GAL4-A101}

(Llorens et al., 2007)

short lived, with Scer\GAL4^repo.PU

(Navarro et al., 2010)

short lived | nutrition conditional

(Navarro et al., 2015)

some die during pupal stage, with Scer\GAL4^da.G32

(Llorens et al., 2007)

some die during pupal stage, with Scer\GAL4^how-24B

(Llorens et al., 2007)

some die during pupal stage, with Scer\GAL4^Ugt36A1.PK

(Llorens et al., 2007)

some die during pupal stage | heat sensitive, with Scer\GAL4^Act.PU

(Llorens et al., 2007)

viable, with Scer\GAL4^c698a

(Llorens et al., 2007)

viable, with Scer\GAL4^Ddc.PL

(Llorens et al., 2007)

viable, with Scer\GAL4^{neur-GAL4-A101}

(Llorens et al., 2007)

viable, with Scer\GAL4^Toll-6-D42

(Llorens et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act5C.PU display a reduced tolerance for elevated dietary iron. Treatment with sucrose or sodium citrate has no effect on survival. Levels of the iron sulphur cluster enzyme aconitase activity are reduced compared with control flies. The reduction in complex II activity in control flies in response to elevated iron levels is more severe upon expression of fh^{dsRNA.Scer\UAS.cLa}. Citrase synthase activity is unaffected.

(Navarro et al., 2015)

Flies expressing flies expressing >fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act.PU are short lived compared to controls. Early treatment with iron chelator deferiprone (DFP) moderately improves survival of flies expressing Scer\GAL4^Act.PU>fh^{dsRNA.Scer\UAS.cLa}. Early treatment with DFP also results in improved climbing ability in flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act.PU Scer\GAL4^{neur-GAL4-A101}.

Idebenone (IDE) is administration to one-day post-eclosion adults improves life span of flies expressing Scer\GAL4^Act.PU>fh^{dsRNA.Scer\UAS.cLa}. Early treatment does not result any significant changes in life span. The impaired climbing ability of Scer\GAL4^Act.PU>fh^{dsRNA.Scer\UAS.cLa} is improved during the second week after IDE treatment.

(Soriano et al., 2013)

Expression of fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^repo.PU results in reduced lifespan, decreased survival under hyperoxia conditions as well as impaired climbing ability in adult flies.

(Navarro et al., 2010)

Expression of fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^{neur-GAL4-A101} results in a statistically significant reduction in life span compared to controls (expression under the control of either Scer\GAL4^D42, Scer\GAL4^c698a or Scer\GAL4^Ddc.PL has no effect on life span).

Expression of fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act.PU at 25[o]C results viable adults which show a reduction of mean and maximum life span of 60% and 32% respectively compared to controls under normoxia. In addition, 45% of 5 day old flies expressing fh^{dsRNA.Scer\UAS.cLa} under these conditions show a reduction in climbing ability compared to controls.

Adults expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act.PU at 25[o]C under hyperoxia (99.5% O[[2]]) show a 44% reduction in average maximum life span compared to controls.

Mitochondria isolated from 1-day old adults expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act.PU at 25[o]C show normal state 3 (active respiration state) and state 4 (basal respiration state) oxygen consumption rates under normoxic conditions. Under hyperoxia, the mutant mitochondria show a two-fold reduction in state 3 oxygen consumption rates compared to controls. Oxygen consumption rates after stimulation of complex I of after stimulation of complex II are the same in the mutant and control mitochondria under both normoxic and hyperoxic conditions.

(Llorens et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fh^RNAi.UAS.cLa has short lived | nutrition conditional phenotype, enhanceable by Scer\GAL4^Act5C.PU/mfrn^UAS.cNa

(Navarro et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PU, fh^RNAi.UAS.cLa has chemical sensitive phenotype, suppressible | partially by Fer3HCH^UAS.cMa, Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

Scer\GAL4^Act5C.PU, fh^RNAi.UAS.cLa has chemical sensitive phenotype, suppressible | partially by Fer2LCH^UAS.cMa/Fer1HCH^UAS.cMa, Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

fh^RNAi.UAS.cLa has short lived phenotype, suppressible by Scer\GAL4^Act5C.PU/Fer3HCH^UAS.cMa

(Navarro et al., 2015)

Scer\GAL4^Act5C.PU, fh^RNAi.UAS.cLa has chemical sensitive phenotype, suppressible | partially by mfrn^GD336/Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

fh^RNAi.UAS.cLa has short lived | nutrition conditional phenotype, suppressible | partially by mfrn^GD336/Scer\GAL4^Act5C.PU

(Navarro et al., 2015)

fh^RNAi.UAS.cLa has short lived | nutrition conditional phenotype, suppressible by Scer\GAL4^Act5C.PU/Fer2LCH^UAS.cMa/Fer1HCH^UAS.cMa

(Navarro et al., 2015)

fh^RNAi.UAS.cLa has short lived phenotype, suppressible by Scer\GAL4^Act5C.PU/Fer2LCH^UAS.cMa/Fer1HCH^UAS.cMa

(Navarro et al., 2015)

fh^RNAi.UAS.cLa has short lived | nutrition conditional phenotype, suppressible by Scer\GAL4^Act5C.PU/Fer3HCH^UAS.cMa

(Navarro et al., 2015)

Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa has abnormal oxidative stress response | adult stage phenotype, suppressible | partially by GLaz^UAS.cWa, Scer\GAL4^repo.PU

(Navarro et al., 2010)

Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa has short lived phenotype, suppressible | partially by GLaz^UAS.cWa, Scer\GAL4^repo.PU

(Navarro et al., 2010)

Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa has abnormal locomotor behavior phenotype, suppressible | partially by GLaz^UAS.cWa, Scer\GAL4^repo.PU

(Navarro et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Fer3HCH^Scer\UAS.cMa partially suppresses the reduction in lifespan seen in flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act5C.PU when fed either normal food or food supplemented with iron. The reduced aconitase levels are not restored by Fer3HCH^Scer\UAS.cMa.

Co-expression of Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa partially suppresses the reduction in lifespan seen in flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act5C.PU when fed either normal food or food supplemented with iron. The reduced aconitase levels are not restored.

Expression of mfrn^GD336 suppresses the reduced survival seen when fh^{dsRNA.Scer\UAS.cLa} is expressed under the control of Scer\GAL4^Act5C.PU in response to 2.5mM iron treatment. The reduced survival seen in fh^{dsRNA.Scer\UAS.cLa}-expressing flies at 15mM iron treatment is not suppressed by mfrn^Scer\UAS.cNa. The reduction in aconitase activity is completely restored.

Expression of mfrn^GD336 enhances the reduction in lifespan seen when fh^{dsRNA.Scer\UAS.cLa} is expressed under the control of Scer\GAL4^Act5C.PU on a normal diet.

Expression of mfrn^Scer\UAS.cNa enhances the reduction in lifespan seen when fh^{dsRNA.Scer\UAS.cLa} is expressed under the control of Scer\GAL4^Act5C.PU in response to elevated dietary iron. Aconitase activity is unaffected. Lifespan reduction is also enhanced on normal food.

(Navarro et al., 2015)

The reduced lifespan, decreased survival under hyperoxia conditions as well as the impaired climbing ability characteristic for adult flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^repo.PU is partially restored by co-expression of GLaz^Scer\UAS.cWa.

(Navarro et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fh^RNAi.UAS.cLa

fh^{dsRNA.Scer\UAS.cLa}

fh^{dsRNA.UAS.cLa}

Name Synonyms

Secondary FlyBase IDs

References (8)

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