Lipodystrophy is characterised by reduced lipid storage in adipose tissue and ectopic lipid storage in non-adipose tissue. Seipinnull larvae show reduced lipid storage in fat bodies and ectopic lipid droplets in the salivary glands.
mdyQX25 Seipinnull double mutant larvae show greatly reduced fat body lipid storage compared to either single mutant (an exacerbation of the lipodystrophy model in adipose tissue). However, the ectopic lipid storage phenotype seen in non-adipose tissue in Seipinnull single mutants is fully suppressed by mdyQX25 (a suppression of the lipodystrophy model in non-adipose tissue).
Overexpression of mdy (using mdyEY07280) restores fat body lipid storage levels to near wild-type in Seipinnull larvae (a suppression of the lipodystrophy model in adipose tissue). However, overexpression of mdy (using mdyEY07280) increases the ectopic lipid storage phenotype observed in non-adipose tissue in Seipinnull larvae (an exacerbation of the lipodystrophy model in non-adipose tissue).
Expression of Ca-P60AScer\UAS.T:Hsap\MYC partially rescues the reduction in lipid droplet size seen in Seipinnull mutant fat body cells. The reduction in ionomycin-evoked cytosolic calcium release is also partially rescued.
The reduction in average weight of Seipinnull flies can be fully rescued by expressing SeipinScer\UAS.cTa with Scer\GAL4tub.PU and partially rescued by expressing SeipinScer\UAS.cTa with Scer\GAL4ppl.PP.
Expression of SeipinScer\UAS.cTa using Scer\GAL4Sgs3.PD rescues the ectopic lipid droplet phenotype in the salivary gland but not the lipid storage defects in the fat body or the ectopic lipid storage in the gut of Seipinnull mutants.
Expression of SeipinΔC.Scer\UAS using Scer\GAL4ppl.PP fully rescues the reduced lipid storage phenotype in the fat body of Seipinnull mutants, but does not rescue the ectopic lipid droplet phenotype in the salivary gland.