Allele Dmel\Seipin^null

General Information
Symbol	Dmel\Seipin^null	Species	D. melanogaster
Name		FlyBase ID	FBal0260636
Feature type	allele	Associated gene	Dmel\Seipin

Allele class	amorphic allele - molecular evidence
Mutagen	ends-out gene targeting, FLPase, SCEI endonuclease
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Tian et al., 2011)
Mutagen	ends-out gene targeting (Tian et al., 2011) FLPase (Tian et al., 2011) SCEI endonuclease (Tian et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference An ends-out gene targeting event in which the whole genomic region of Seipin is replaced with Avic\GFP and w[+mC]. (Tian et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Tian et al., 2011) lethal \| nutrition conditional (Tian et al., 2011) viable (Tian et al., 2011)
Phenotype Manifest In
	adult fat body \| nutrition conditional (Tian et al., 2011) embryonic/larval fat body \| nutrition conditional (Tian et al., 2011) fat body (Tian et al., 2011) lipid particle (Tian et al., 2011) lipid particle \| ectopic (Tian et al., 2011) salivary gland (Tian et al., 2011)
Detailed Description
	Statement Reference Mutants have no noticeable behaviour defects. Fat bodies of Seipin[null] larvae and fat cells from young Seipin[null] adults have significantly reduced lipid storage compared to wild type. This small lipid droplet phenotype is more sever under starvation conditions. The overall morphology of the fat body in Seipin[null] mutants appears normal. Total glyceride content in Seipin[null] mutants is greatly reduced compared to controls. The average weight of Seipin[null] flies is lower than controls. Under fed conditions, the amount of neutral lipids in oenocytes of Seipin[null] animals is the same as wild type. Neutral lipids are higher in starved Seipin[null] animals, but sill much lower than wild type. Circulating glyceride levels in the hemolymph of Seipin[null] mutants are much lower than in wild type. Under starved conditions, 100% of Seipin[null] flies die within 42 hours, whereas more than half of control flies survive for 48 hours. The proventriculus and anterior midgut region of Seipin[null] mutants contain abnormally large lipid droplets and more stored lipid, respectively, compared to controls. Seipin[null] mutants exhibit ectopic lipid droplets in the salivary gland, which normally lacks any visible lipid droplets. Seipin[null] mutant larvae have increased levels of total and most individual species of phosphatidic acid. The salivary glands of Seipin[null] mutant larvae have increased levels of total and several species of diacylglycerol. In Seipin[null] mutants, the level of triacylglycerol (TAG) is greatly reduced in the fat body, while the levels of several TAG species are significantly increased in salivary glands. (Tian et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Seipin^null has fat body phenotype, enhanceable by mdy^QX25 (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, enhanceable by CdsA¹ (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, enhanceable by CG5508^EY00734/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, enhanceable by mdy^EY07280/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle phenotype, enhanceable by mdy^QX25 (Tian et al., 2011) Seipin^null has salivary gland phenotype, enhanceable by CdsA¹ (Tian et al., 2011) Seipin^null has salivary gland phenotype, enhanceable by CG5508^EY00734/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, enhanceable by mdy^EY07280/Scer\GAL4^48Y (Tian et al., 2011)
NOT Enhanced by
Statement Reference Seipin^null has fat body phenotype, non-enhanceable by Lsd-2^KG00149 (Tian et al., 2011) Seipin^null has lipid particle phenotype, non-enhanceable by Lsd-2^KG00149 (Tian et al., 2011)
Suppressed by
Statement Reference Seipin^null has fat body phenotype, suppressible by Hsap\BSCL2^{S90L.Scer\UAS}/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has fat body phenotype, suppressible by mdy^EY07280/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has fat body phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^Scer\UAS.cTa (Tian et al., 2011) Seipin^null has fat body phenotype, suppressible by Scer\GAL4^ppl.PP/SCAP^EY06708 (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by CdsA^EY08412/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by Hsap\BSCL2^{S90L.Scer\UAS}/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by Lpin^GD14004/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by Lpin^GD14004/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by mdy^QX25 (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^Scer\UAS.cTa (Tian et al., 2011) Seipin^null has lipid particle phenotype, suppressible by Hsap\BSCL2^{S90L.Scer\UAS}/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has lipid particle phenotype, suppressible by mdy^EY07280/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has lipid particle phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^Scer\UAS.cTa (Tian et al., 2011) Seipin^null has lipid particle phenotype, suppressible by Scer\GAL4^ppl.PP/SCAP^EY06708 (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by CdsA^EY08412/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by Hsap\BSCL2^{S90L.Scer\UAS}/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by Lpin^GD14004/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by Lpin^GD14004/Scer\GAL4^ppl.PP (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by mdy^QX25 (Tian et al., 2011) Seipin^null has salivary gland phenotype, suppressible by Scer\GAL4^ppl.PP/Hsap\BSCL2^Scer\UAS.cTa (Tian et al., 2011)
NOT suppressed by
Statement Reference Seipin^null has lipid particle \| ectopic phenotype, non-suppressible by CG3812^GD3545/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, non-suppressible by CG5508^e01407a (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, non-suppressible by fu12^GD3463/CG3812^GD3545/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has lipid particle \| ectopic phenotype, non-suppressible by fu12^GD3463/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, non-suppressible by CG3812^GD3545/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, non-suppressible by CG5508^e01407a (Tian et al., 2011) Seipin^null has salivary gland phenotype, non-suppressible by fu12^GD3463/CG3812^GD3545/Scer\GAL4^48Y (Tian et al., 2011) Seipin^null has salivary gland phenotype, non-suppressible by fu12^GD3463/Scer\GAL4^48Y (Tian et al., 2011)
Enhancer of
Statement Reference Seipin^null is an enhancer of fat body phenotype of mdy^QX25 (Tian et al., 2011) Seipin^null is an enhancer of lipid particle \| ectopic phenotype of CdsA¹ (Tian et al., 2011) Seipin^null is an enhancer of salivary gland phenotype of CdsA¹ (Tian et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Fat body lipid storage in Seipin[null]; Lsd-2[KG00149] animals is similar to Seipin[null] single mutants. mdy[QX25], Seipin[null] mutant fat bodies show greatly reduced lipid storage compared to either single mutant. Expression of either mdy[EY07280] or SCAP[EY06708] using Scer\GAL4[ppl.PP] suppresses the reduced lipid storage phenotype seen in Seipin[null] fat bodies. Overexpression of CG5508[EY00734] using Scer\GAL4[48Y] enhances the ectopic lipid storage phenotype in salivary glands of Seipin[null] mutants. Overexpression of mdy[EY07280] using Scer\GAL4[48Y] in a Seipin[null] mutant background results in a massive ectopic lipid storage phenotype in salivary glands. The ectopic lipid storage phenotype of Seipin[null] mutants is fully suppressed by mdy[QX25] or by expression of Lpin[GD14004] using either Scer\GAL4[ppl.PP] or Scer\GAL4[48Y]. The ectopic lipid storage phenotype of Seipin[null] mutants is not suppressed by CG5508[e01407a] or by expression of fu12[GD3463], CG3812[GD3545] or fu12[GD3463] and CG3812[GD3545] together using Scer\GAL4[48Y]. CdsA[1], Seipin[null] double mutants exhibit a strong synergistic phenotype (compared to either single mutant) with respect to ectopic lipid droplets in the salivary gland. The ectopic lipid storage phenotype of Seipin[null] mutants is fully suppressed by expressing CdsA[EY08412] using Scer\GAL4[48Y]. There is more phosphatidic acid in the salivary glands of CdsA[1]; Seipin[null] mutants than in CdsA[1] single mutants. (Tian et al., 2011)
Xenogenetic Interactions
Statement Reference Lipid storage in the fat body is restored and ectopic lipid storage in the salivary gland is reversed in Seipin[null] mutants with Hsap\BSCL2[Scer\UAS.cTa] or Hsap\BSCL2[S90L.Scer\UAS] driven by Scer\GAL4[ppl.PP]. (Tian et al., 2011)
Complementation & Rescue Data
Rescued by	Seipin^null is rescued by Scer\GAL4^Lsp2.PH/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is rescued by Scer\GAL4^ppl.PP/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is rescued by Scer\GAL4^Sgs3.PD/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is rescued by Scer\GAL4^tub.PU/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is rescued by Seipin^ΔC.Scer\UAS/Scer\GAL4^ppl.PP (Tian et al., 2011)
Partially rescued by	Seipin^null is partially rescued by Scer\GAL4^ppl.PP/Seipin^Scer\UAS.cTa (Tian et al., 2011)
Not rescued by	Seipin^null is not rescued by Scer\GAL4^Lsp2.PH/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is not rescued by Scer\GAL4^Sgs3.PD/Seipin^Scer\UAS.cTa (Tian et al., 2011) Seipin^null is not rescued by Seipin^ΔC.Scer\UAS/Scer\GAL4^ppl.PP (Tian et al., 2011)
Comments	The reduction in total glyceride content of Seipin[null] is rescued by expression of Seipin[Scer\UAS.cTa] using Scer\GAL4[tub.PU]. The reduction in average weight of Seipin[null] flies can be fully rescued by expressing Seipin[Scer\UAS.cTa] with Scer\GAL4[tub.PU] and partially rescued by expressing Seipin[Scer\UAS.cTa] with Scer\GAL4[ppl.PP]. Expression of Seipin[Scer\UAS.cTa] using Scer\GAL4[tub.PU] reverts the starvation hypersensitivity of Seipin[null] flies to control levels. Expression of Seipin[Scer\UAS.cTa] using Scer\GAL4[ppl.PP] rescues the lipid storage defects in the fat body and ectopic lipid storage in the salivary gland of Seipin[null] mutants. Expression of Seipin[Scer\UAS.cTa] using Scer\GAL4[Lsp2.PH] rescues the lipid storage defects in the fat body but not in the salivary gland or in the gut of Seipin[null] mutants. Expression of Seipin[Scer\UAS.cTa] using Scer\GAL4[Sgs3.PD] rescues the ectopic lipid droplet phenotype in the salivary gland but not the lipid storage defects in the fat body or the ectopic lipid storage in the gut of Seipin[null] mutants. Expression of Seipin[ΔC.Scer\UAS] using Scer\GAL4[ppl.PP] fully rescues the reduced lipid storage phenotype in the fat body of Seipin[null] mutants, but does not rescue the ectopic lipid droplet phenotype in the salivary gland. (Tian et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Seipin^null
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Tian et al., 2011, PLoS Genet. 7(4): e1001364 Tissue-autonomous function of Drosophila seipin in preventing ectopic lipid droplet formation. [FBrf0213596]

Allele Dmel\Seipinnull

Allele Dmel\Seipin^null