FlyBase Allele Report: Dmel\Nup44A[Δ15]

General Information

Symbol

Dmel\Nup44A^Δ15

Species

D. melanogaster

Name

FlyBase ID

FBal0260725

Feature type

allele

Associated gene

Dmel\Nup44A

Associated Insertion(s)

Carried in Construct

Also Known As

seh1^Δ15

Genomic Maps

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Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Senger et al., 2011)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

2R:7,989,444..7,992,219 [+]

Comment:

2776 bp deletion generated by site-directed recombination reported as extending from 2R: 3,876,949 to 3,879,724 in Release 5 coordinates.

(Senger et al., 2011)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{RS3}Nup44A^CB-0750-3

(Senger et al., 2011)

PBac{WH}f07552

(Senger et al., 2011)

Cytology

Nature of the lesion

Statement

Reference

Flipase recognition target (Scer\FRT) site directed recombination between PBac{WH}f07552 and P{RS3}Nup44A^CB-0750-3 generated a 2775 bp deletion encompassing Nup44A coding sequences.

(Senger et al., 2011)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased fecundity | female | recessive

(Senger et al., 2011)

female semi-sterile | recessive

(Senger et al., 2011)

viable | recessive

(Senger et al., 2011)

Phenotype Manifest In

autolysosome (with Df(2R)ED1735)

(Wei et al., 2014)

centriole

(Senger et al., 2011)

egg chamber

(Wei et al., 2014, Senger et al., 2011)

egg chamber (with Df(2R)ED1735)

(Wei et al., 2014)

germarium

(Senger et al., 2011)

microtubule

(Senger et al., 2011)

oocyte

(Senger et al., 2011)

oocyte (with Df(2R)ED1735)

(Senger et al., 2011)

ovary

(Cai et al., 2016)

Detailed Description

Statement

Reference

Nup44A^Δ15/Nup44A^Δ15 larval fat bodies do not show inappropriate accumulation of autolysosomes. Nup44A^Δ15/Nup44A^Δ15 ovaries show accumulation of acidic puncta.

(Cai et al., 2016)

Ovarian cysts, but not fat bodies, from Nup44A^Δ15/Df(2R)ED1735 animals accumulate autolysosomes.

Egg chambers from Nup44A^Δ15 females often develop without an oocyte and cease to develop beyond stage 5 or 6 of oogenesis.

(Wei et al., 2014)

Nup44A^Δ15 mutants are homozygous viable and eclose nearly at the expected Mendelian ratio. However Nup44A^Δ15 homozygous females exhibit markedly reduced fecundity, laying just one-quarter the eggs of wild-type controls. The eggs laid by Nup44A^Δ15 mutant females hatch at about 40% of the rate of those laid by age-matched wild-type controls.Consistent with reduced fertility, Nup44A^Δ15 mutant ovarioles contain fewer egg chambers and exhibit smaller germaria than ovarioles from wild-type females.

Nup44A^Δ15 mutant germaria contain wild-type numbers of mitotic ovarian cysts but have a reduction in the number of post-mitotic cysts.

Approximately 20% of Nup44A^Δ15 homozygous and Nup44A^Δ15/Df(2R)ED1735 transheterozygous mutant egg chambers develop with 16 polyploid nurse cells and no oocyte.

In a fraction of Nup44A^Δ15 mutant egg chambers, the centrioles fail to migrate to the posterior of the oocyte. In the mutant egg chambers, the distribution of microtubules is frequently disorganised.

Homozygous Nup44A^Δ15 mutants exhibit a mitotic delay during the ovarian cyst division.

(Senger et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Nup44A^Δ15 has egg chamber phenotype, enhanceable by mio²

(Senger et al., 2011)

Suppressed by

Statement

Reference

Nup44A^Δ15 has egg chamber phenotype, suppressible by Scer\GAL4^nos.PG/Nprl2^{dsRNA.shRNA.UAS.V20}

(Wei et al., 2014)

Nup44A^Δ15 has egg chamber phenotype, suppressible by Scer\GAL4^nos.PG/Nprl3^{dsRNA.shRNA.UAS.V20}

(Wei et al., 2014)

Nup44A^Δ15 has egg chamber phenotype, suppressible by Scer\GAL4^nos.PG/Tsc1^GL00012

(Wei et al., 2014)

NOT suppressed by

Statement

Reference

Nup44A^Δ15 has ovary phenotype, non-suppressible by Scer\GAL4^VP16.nos.UTR/Nprl2^{dsRNA.shRNA.UAS.V20}

(Cai et al., 2016)

Nup44A^Δ15 has ovary phenotype, non-suppressible by Scer\GAL4^VP16.nos.UTR/Nprl3^{dsRNA.shRNA.UAS.V20}

(Cai et al., 2016)

Suppressor of

Statement

Reference

Nup44A^Δ15/Nup44A[+] is a suppressor of egg chamber phenotype of mio²/mio¹

(Senger et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nprl2^{dsRNA.shRNA.Scer\UAS} or Nprl3^{dsRNA.shRNA.Scer\UAS} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress accumulation of acidic puncta in Nup44A^Δ15/Nup44A^Δ15 ovaries.

(Cai et al., 2016)

The percentage of mio¹/mio² mutant egg chambers with an oocyte increases 6-fold in a Nup44A^Δ15/+ genetic background.

Ovaries from mio², Nup44A^Δ15 double mutant females closely resemble those from Nup44A^Δ15 single mutants, with 80% or egg chambers containing an oocyte and 20% containing 16 nurse cells and no obvious oocyte. However, ovaries from mio², Nup44A^Δ15 double homozygotes have fewer older egg chambers than Nup44A^Δ15 single mutants.

(Senger et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nup44A^Δ15 is rescued by Nup44A^{UASp.mCer,Tag:HA}/Scer\GAL4^VP16.nos.UTR

(Senger et al., 2011)

Comments

The reductions in Nup44A^Δ15 fertility, as well as the specific oogenesis defects are rescued when Nup44A^{Scer\UAS.P\T.T:Avic\GFP-ECFP.Cer,T:Ivir\HA1} is expressed using the germline-specific driver Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Senger et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nup44A^Δ15

seh1^Δ15

(Yang et al., 2020, Kim et al., 2017, Cai et al., 2016, Wei et al., 2014, Senger et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (5)

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