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General Information
Symbol
Dmel\NetBtm
Species
D. melanogaster
Name
FlyBase ID
FBal0260876
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A transmembrane domain (Tag:TM(Synth)) flanked by Tag:V5 (intracellular) and Tag:MYC (extracellular) epitope tags has been introduced to the C-terminus of NetB. The transmembrane domain is expected to tether the NetB protein to the cell membrane.

Insertion components
TI{TI}NetBtm
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

The tagged membrane-bound NetB is homogenously expressed in all tin-positive cardoblast membranes but not in tinFBtr0091350:pb-XRsvp-expressing cells along the dorsal vessel.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A small percentage of R8 axons in NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC mutants project abnormally, past the distal medulla neuropil border at 24 and 42 hour after pupal formation. Projections are unaffected at 55 hours and in adults.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The anterior commissure appears normal in all segments and the posterior commissure appears normal in most of the segments in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC double mutant embryos. Similarly to wild-type, both the EW and EG axons cross the midline in every segment of NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos.

NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC animals are viable and fertile with no obvious morphological phenotype.

Short-range repulsion is preserved in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL41407, as the ventral nerve cord commissures are completely absent in every segment examined.

Long-range axon-repulsion is completely abolished in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC
Name Synonyms
Secondary FlyBase IDs
    References (6)