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General Information
Symbol
Dmel\G9aDD2
Species
D. melanogaster
Name
FlyBase ID
FBal0260882
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EHMTDD2
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

1473 bp deletion resulting from the imprecise excsion of P{SUPor-P}G9aKG01242 removes the G9a translation start site.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imperfect excision of P{SUPor-P}G9aKG01242, resulting in loss of 1473 bp of DNA downstream of the insertion site which includes the translational start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The average life span of G9aDD2 is slightly longer than that of wild-type controls.

G9aDD2 mutant flies show increased sensitivity to infection by Drosophila C virus (DCV), Cricket paralysis virus (CrPV), Flock House Virus (FHV), Drosophila X Virus (DXV) relative to wild-type. The mutant flies show reduced survival after RNA virus infection compared with wild-type. Survival rates of G9aDD2 mutants after infection with the DNA virus Invertebrate iridescent virus 6 (IIV-6) are similar to wild-type. No significant difference is detected in DCV viral titers between mutant and wild-type flies, indicating that G9aDD2 mutants show reduced tolerance to RNA virus infection.

General nervous system development and neuronal function is not affected in mutant flies.

Dendrite development in multidendrite (md) neurons is altered in mutant flies. While the basic organization of dendritic arbours in type 4 md neurons is maintained, there is a reduction of higher order branching resulting in dendritic fields of appreciably reduced complexity. The total number of dendrite ends is significantly reduced.

The total path length covered by foraging mutant larvae is not different from controls. However, mutant larvae often stop, retract and turn, causing increased branching in their crawling paths.

Adult phototaxis and geotaxis behaviours are normal in mutants.

Hemizygous males and G9aDD1/G9aDD2 females both display a drastically slower response decrement during habituation to a "light-off jump reflex" compared to controls.

Short-term courtship memory of G9aDD2 males is reduced compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Suppressor of
Statement
Reference

G9aDD2 is a non-suppressor of eye phenotype of Diap1dsRNA.UAS, Scer\GAL4GMR.PF

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

The early lethality of DCV infected G9aDD2 mutants is rescued to control levels by fat body-specific expression of G9aScer\UAS.cKa under the control of Scer\GAL4c564.

Expression of G9aScer\UAS.cKa under the control of either Scer\GAL4Hml.PG or Scer\GAL4repo fails to rescue the hypersensitivity of G9aDD2 mutant flies to DCV infection.

Expression of G9aScer\UAS.cKa using Scer\GAL4477 in a G9aDD2 background rescues dendrite branching towards wild type levels.

Expression of G9aScer\UAS.cKa using Scer\GAL4477 in a G9aDD2 background fails to restore normal larval locomotor behaviour.

Expression of G9aScer\UAS.cKa using Scer\GAL4elav.PLu or Scer\GAL47B restores the short-term courtship memory defect of G9aDD2 males.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)