G9aDD2 homozygous adults exhibit increased mortality upon systematic infection with a range of doses of Drosophila C Virus (DCV) compared to controls. When challenged with the highest dose (10) mortality is similar between mutants and controls. The viral load increases in a dose-dependent manner in both mutants and controls. Female flies achieve higher viral loads measured 5 days post-DCV-infection compared to males and the sensitivity to infection difference between mutants and controls is much higher in females than males. According to nonlinear 4-parameter logistic model, mutants are more sensitive to increasing viral doses while there is no difference between mutants and controls in the severity of infection.
The average life span of G9aDD2 is slightly longer than that of wild-type controls.
G9aDD2 mutant flies show increased sensitivity to infection by Drosophila C virus (DCV), Cricket paralysis virus (CrPV), Flock House Virus (FHV), Drosophila X Virus (DXV) relative to wild-type. The mutant flies show reduced survival after RNA virus infection compared with wild-type. Survival rates of G9aDD2 mutants after infection with the DNA virus Invertebrate iridescent virus 6 (IIV-6) are similar to wild-type. No significant difference is detected in DCV viral titers between mutant and wild-type flies, indicating that G9aDD2 mutants show reduced tolerance to RNA virus infection.
General nervous system development and neuronal function is not affected in mutant flies.
Dendrite development in multidendrite (md) neurons is altered in mutant flies. While the basic organization of dendritic arbours in type 4 md neurons is maintained, there is a reduction of higher order branching resulting in dendritic fields of appreciably reduced complexity. The total number of dendrite ends is significantly reduced.
The total path length covered by foraging mutant larvae is not different from controls. However, mutant larvae often stop, retract and turn, causing increased branching in their crawling paths.
Adult phototaxis and geotaxis behaviours are normal in mutants.
Hemizygous males and G9aDD1/G9aDD2 females both display a drastically slower response decrement during habituation to a "light-off jump reflex" compared to controls.
Short-term courtship memory of G9aDD2 males is reduced compared to controls.