Allele Dmel\gsb^{491.dsRNA.Scer\UAS}

General Information
Symbol	Dmel\gsb^{491.dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0260925
Feature type	allele	Associated gene	Dmel\gsb

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Marie et al., 2010) in vitro construct - RNAi (Marie et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of 491bp of gsb coding sequence in an inverted repeat, generating dsRNA. (Marie et al., 2010)
Carried in construct	P{UAS-gsb-RNAi.491} (Marie et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	wing, with Scer\GAL4^Bx-MS1096 (Marie et al., 2010)
Detailed Description
	Statement Reference Overexpression of gsb[491.dsRNA.Scer\UAS] in the developing wing under the control of Scer\GAL4[Bx-MS1096] results in a severe perturbation of wing morphology. Overexpression of gsb[491.dsRNA.Scer\UAS] in post-mitotic neurons under the control of Scer\GAL4[elav-C155] does not generate a statistically significant difference in mEPSP amplitude and quantal content. Overexpression of gsb[491.dsRNA.Scer\UAS] in post-mitotic neurons under the control of Scer\GAL4[elav-C155] does not alter bouton size or 'satellite' bouton formation in mutant neuromuscular junctions. (Marie et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference gsb^{491.dsRNA.Scer\UAS}/Scer\GAL4^C57 is a non-enhancer of neurophysiology defective phenotype of GluRIIA^SP16 (Marie et al., 2010) Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a non-enhancer of neurophysiology defective phenotype of Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa (Marie et al., 2010)
Suppressor of
Statement Reference Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a suppressor of neurophysiology defective phenotype of GluRIIA^SP16 (Marie et al., 2010)
NOT Suppressor of
Statement Reference gsb^{491.dsRNA.Scer\UAS}/Scer\GAL4^C57 is a non-suppressor of neurophysiology defective phenotype of GluRIIA^SP16 (Marie et al., 2010) Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a non-suppressor of neurophysiology defective phenotype of Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa (Marie et al., 2010)
Phenotype Manifest In
NOT Enhancer of
Statement Reference gsb^{491.dsRNA.Scer\UAS}/Scer\GAL4^C57 is a non-enhancer of neuromuscular junction phenotype of GluRIIA^SP16 (Marie et al., 2010) Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a non-enhancer of neuromuscular junction phenotype of Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa (Marie et al., 2010)
Suppressor of
Statement Reference Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a suppressor of neuromuscular junction phenotype of GluRIIA^SP16 (Marie et al., 2010)
NOT Suppressor of
Statement Reference gsb^{491.dsRNA.Scer\UAS}/Scer\GAL4^C57 is a non-suppressor of neuromuscular junction phenotype of GluRIIA^SP16 (Marie et al., 2010) Scer\GAL4^elav-C155/gsb^{491.dsRNA.Scer\UAS} is a non-suppressor of neuromuscular junction phenotype of Scer\GAL4^elav-C155, VGlut^Scer\UAS.cDa (Marie et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of gsb[491.dsRNA.Scer\UAS] under the control of Scer\GAL4[elav-C155] in a GluRIIA[SP16] heterozygous background significantly suppresses the homeostatic increase in presynaptic release normally observed in GluRIIA[SP16] mutants. Expression of gsb[491.dsRNA.Scer\UAS] under the control of Scer\GAL4[C57] does not change the synaptic homeostasis found in GluRIIA[SP16] heterozygotes. Expression of gsb[491.dsRNA.Scer\UAS] under the control of Scer\GAL4[elav-C155] in a GluRIIA[SP16] heterozygous background significantly reduces the synaptic depression normally observed in GluRIIA[SP16] mutants. Expression of VGlut[Scer\UAS.cDa] and gsb[491.dsRNA.Scer\UAS] in post-mitotic neurons under the control of Scer\GAL4[elav-C155] causes a significant increase in average mEPSP amplitude. There is also a homeostatic decrease in presynaptic quantal content in these mutants. (Marie et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	gsb^{491.dsRNA.Scer\UAS} is rescued by gsb^Scer\UAS.cMa/Scer\GAL4^Bx-MS1096 (Marie et al., 2010)
Comments	Co-expression of gsb[Scer\UAS.cMa] with gsb[491.dsRNA.Scer\UAS] is able to significantly suppress the wing morphological defect found upon expression of gsb[491.dsRNA.Scer\UAS] alone. (Marie et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	gsb^{491.dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Marie et al., 2010, J. Neurosci. 30(24): 8071--8082 Synaptic homeostasis is consolidated by the cell fate gene gooseberry, a Drosophila pax3/7 homolog. [FBrf0211066]

Allele Dmel\gsb491.dsRNA.Scer\UAS

Allele Dmel\gsb^{491.dsRNA.Scer\UAS}