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General Information
Symbol
Dmel\Cip41
Species
D. melanogaster
Name
FlyBase ID
FBal0260999
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dcip41
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EP}Cip4EP3507 generates a deletion that removes part of the Cip41 first exon including the initiation codon, the first intron, and the second exon of the Cip4 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar Cip41/Df(3L)ED4342 larvae show an increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

Cip41 homozygotes and Cip41/Df(3L)ED4342 trans-heterozygotes are semi-lethal at the pupal stage with an incidence of lethality of ~17%.

Cip41 mutant larvae exhibit an increase in bouton number, satellite bouton number and neuromuscular junction length.

Compared with wild-type controls, Cip41/Df(3L)ED4342 trans-heterozygous mutant larvae exhibit an increase in bouton number normalized to muscle surface area of approximately 32%. In addition, satellite bouton number and neuromuscular junction length normalized to muscle surface area are increased by 67% and 24%, respectively.

Cip41 heterozygous neuromuscular junctions do not exhibit any morphological changes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Cip41 has abnormal neuroanatomy phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp1/Cdc422

Cip41 has abnormal neuroanatomy phenotype, enhanceable by Cdc42[+]/Cdc422

Cip41 has abnormal neuroanatomy phenotype, enhanceable by WASp[+]/WASp1

Cip41 has abnormal neuroanatomy phenotype, enhanceable by Arp3EP3640/Arp66B[+]

NOT suppressed by
Enhancer of
Statement
Reference

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of abnormal neuroanatomy phenotype of WASp1

Cip41/Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Cdc422

Cip41, WASp[+], WASp1, Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Cdc422

Cip41/Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Arp3EP3640

Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

Cip41 has NMJ bouton phenotype, enhanceable by Cdc42[+]/Cdc422

Cip41 has neuromuscular junction phenotype, enhanceable by Cdc42[+]/Cdc422

Cip41 has neuromuscular junction phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp1/Cdc422

Cip41 has NMJ bouton phenotype, enhanceable by WASp[+]/WASp1

Cip41 has neuromuscular junction phenotype, enhanceable by WASp[+]/WASp1

Cip41 has NMJ bouton phenotype, enhanceable by Arp3EP3640/Arp66B[+]

Cip41 has neuromuscular junction phenotype, enhanceable by Arp3EP3640/Arp66B[+]

Cip41 has NMJ bouton phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp1/Cdc422

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Cip41/Cip4[+] is an enhancer of NMJ bouton phenotype of WASp1

Cip41/Cip4[+] is an enhancer of neuromuscular junction phenotype of WASp1

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of NMJ bouton phenotype of WASp1

Cdc42[+], Cip41, Cip4[+], Cdc422 is an enhancer of neuromuscular junction phenotype of WASp1

Cip41/Cip4[+] is an enhancer of NMJ bouton phenotype of Cdc422

Cip41/Cip4[+] is an enhancer of neuromuscular junction phenotype of Cdc422

Cip41, WASp[+], WASp1, Cip4[+] is an enhancer of NMJ bouton phenotype of Cdc422

Cip41, WASp[+], WASp1, Cip4[+] is an enhancer of neuromuscular junction phenotype of Cdc422

Cip41/Cip4[+] is an enhancer of NMJ bouton phenotype of Arp3EP3640

Cip41/Cip4[+] is an enhancer of neuromuscular junction phenotype of Arp3EP3640

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar Cip41/Df(3L)ED4342 larvae is not altered if they are also mutant for RhoGAP92B1/RhoGAP92B2.

The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B1/RhoGAP92B2 larvae is significantly suppressed by Cip41/+.

Cdc422; Cip41 double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

WASp1/Cip41 double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Arp66BEP3640/Cip41 double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Cdc422/+; Cip41/+; WASp1/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Ubiquitous expression of Cip4Scer\UAS.N.T:Ivir\HA1 under the control of Scer\GAL4da.G32 leads to a nearly complete rescue of Cip41 lethality.

Expression of Cip4Scer\UAS.N.T:Ivir\HA1 in Cip41/Df(3L)ED4342 trans-heterozygotes under the control of the muscle-specific Scer\GAL4C57 fully rescues the synaptic overgrowth phenotype.

Expression of Cip4Scer\UAS.N.T:Ivir\HA1 in Cip41/Df(3L)ED4342 trans-heterozygotes under the control of the neuron-specific Scer\GAL4elav-C155 fails to rescue the synaptic overgrowth phenotype.

Post-synaptic expression of Cip4L145D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4C57 rescues the synaptic overgrowth phenotype found in Cip41/Df(3L)ED4342 trans-heterozygotes.

Post-synaptic expression of Cip4L436S.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4C57 fails to rescue the synaptic overgrowth phenotype found in Cip41/Df(3L)ED4342 trans-heterozygotes.

Post-synaptic expression of Cip4W603K.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4C57 fails to rescue the synaptic overgrowth phenotype found in Cip41/Df(3L)ED4342 trans-heterozygotes.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)