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General Information
Symbol
Dmel\aru8.128
Species
D. melanogaster
Name
FlyBase ID
FBal0261089
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P-element mutagenesis results in a P-element being inserted in the 5' region of aru.

Insertion components
P{GawB}aru8.128
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

aru8.128 flies display a robust increase in ethanol sensitivity. aru8.128 flies are healthy, fertile, and the gross morphology of their brains appear normal. aru8.128 flies exhibit a normal rate of ethanol absorption indicating that their increased sensitivity is not due to altered ethanol pharmacokinetics. However, this increase in ethanol sensitivity can be reversed by adult social isolation.

The time for 50% of aru8.128 mutant flies to reach sedation (ST50) is significantly decreased compared to wild-type controls (in a loss-of-righting reflex assay). aru8.128 mutant flies have a normal right reflex in the absence of ethanol and show normal baseline locomotor activity and startle-induced climbing.

aru8.128 heterozygous mutants show enhanced ethanol sensitivity.

aru8.128 flies exhibit normal circadian rhythms.

aru8.128 mutant larvae show a significant increase in the number of synaptic boutons (by 36.6% compared to controls), whose structure and morphology appear normal. The PDF neurons of adult aru8.128 flies also show a similar increase in synaptic terminals (by 29.8% compared to controls), showing that aru regulates synapse number in both larval and adult neurons. Social isolation of aru8.128 flies normalizes the number of synaptic terminals of PDF neurons.

After conditioning for twenty minutes with 41[o]C negative reinforcement, place memory levels are reduced ~30-35% in aru8.128 flies compared to the performance of canton-S wild-type flies. aru8.128 flies in a Berlin genetic background exhibit a non-significant reduction in memory performance.

aru8.128 flies exhibit a reduced olfactory memory after three minutes following training in a wild-type Berlin genetic background. In this background, aru8.128 flies exhibit a ~40% reduction in memory compared to Berlin flies memory performance. aru8.128 flies in a Canton-S background have memory level that are similar to the Canton-S performance levels. Thus, in olfactory classical conditioning, the aru8.128 allele can influence memory levels, but this depends on the genetic background in which it is tested.

aru8.128 flies exhibit wild-type abilities to sense and avoid odours, electric shock, and high temperatures.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Flies over-expressing rlSem.C.Scer\UAS in neurons with Scer\GAL4elav-C155 in a aru8.128 background shows increased sensitivity to ethanol sedation that is not significantly different from that of aru8.128 flies, indicating that a aru8.128 background suppresses the decreased ethanol sensitivity caused by overexpression of rlSem.C.Scer\UAS.

The presence of a aru8.128 background suppresses the ethanol resistance found in hppy17-51 mutants.

Flies over-expressing Pi3K92EA2860C.Scer\UAS in neurons (with Scer\GAL4elav-C155) in an aru8.128 background show increased ethanol sensitivity, indicating suppression of the Pi3K92EA2860C.Scer\UAS phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)