FlyBase Allele Report: Dmel\Ndfip[UAS.Tag:HA]

General Information

Symbol

Dmel\Ndfip^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0261132

Feature type

allele

Associated gene

Dmel\Ndfip

Associated Insertion(s)

Carried in Construct

P{UAS-Ndfip.HA}

Key Links

Transgenic product class

wild_type

(Dalton et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dalton et al., 2011)

Mutagen

in vitro construct

(Dalton et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Ndfip.HA}

Cytology

Description

UAS regulatory sequences drive expression of Ndfip which is tagged with a Tag:HA epitope.

(Dalton et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ndfip

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing margin bristle | increased number, with Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

Detailed Description

Statement

Reference

Over-expression of a single copy of Ndfip^{Scer\UAS.T:Ivir\HA1} with Scer\GAL4^ptc-559.1 or Scer\GAL4^Bx-MS1096 has minimal effects on the adult wing. However, over-expression of two copies of Ndfip^{Scer\UAS.T:Ivir\HA1} using Scer\GAL4^Bx-MS1096 results in severe wing disruption, with increased bristles present at the wing margin.

(Dalton et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Ndfip^UAS.Tag:HA, Scer\GAL4^ptc-559.1 is an enhancer of wing margin phenotype of Nedd4^GS13178, Scer\GAL4^ptc-559.1

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of Nedd4^GS13178, Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA, Scer\GAL4^ptc-559.1 is an enhancer of wing phenotype of Scer\GAL4^ptc-559.1, Su(dx)^UAS.cCa

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of Scer\GAL4^Bx-MS1096, Su(dx)^UAS.cCa

(Dalton et al., 2011)

Suppressor of

Statement

Reference

Ndfip^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, dx^GD1427

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096 is a suppressor of wing margin phenotype of N^264-39

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096 is a suppressor of wing margin bristle phenotype of N^264-39

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of N^264-39

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096 is a suppressor of wing margin phenotype of Ser¹

(Dalton et al., 2011)

Other

Statement

Reference

Ndfip^UAS.Tag:HA, Nedd4^GS13178, Scer\GAL4^Bx-MS1096 has wing disc phenotype

(Dalton et al., 2011)

Ndfip^UAS.Tag:HA, Scer\GAL4^Bx-MS1096, Su(dx)^UAS.cCa has wing disc phenotype

(Dalton et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Nedd4^GS13178, Scer\GAL4^ptc-559.1 wing phenotype, with severe loss of the wing margin producing smaller wings.

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Su(dx)^Scer\UAS.cCa, Scer\GAL4^ptc-559.1 wing phenotype, with loss of the wing margin.

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Nedd4^GS13178, Scer\GAL4^Bx-MS1096 wing phenotype, with little intervein space evident, thick veins and smaller wings.

Co-expression of Ndfip^{Scer\UAS.T:Ivir\HA1} enhances the Su(dx)^Scer\UAS.cCa, Scer\GAL4^Bx-MS1096 wing phenotype, resulting in a severely disrupted wing with increased vein thickening and smaller wing size.

Wings discs that co-express Su(dx)^Scer\UAS.cCa and Ndfip^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 are undulated, unlike wild type.

Wings discs that co-express Nedd4^GS13178 and Ndfip^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 are quite undulated and wrinkled, unlike wild type.

Scer\GAL4^Bx-MS1096-mediated expression of Ndfip^{Scer\UAS.T:Ivir\HA1} suppresses the N^264-39/+ wing phenotypes.

Coexpression of Ndfip^{Scer\UAS.T:Ivir\HA1} suppresses the Scer\GAL4^Bx-MS1096, dx^GD1427 wing phenotype, reducing the vein thickening.

The Ser¹/+ wing phenotype is suppressed by Scer\GAL4^Bx-MS1096-mediated overexpression of Ndfip^{Scer\UAS.T:Ivir\HA1}.

(Dalton et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ndfip^{Scer\UAS.T:Ivir\HA1}

Ndfip^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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