FB2025_01 , released February 20, 2025
Allele: Dmel\C3GMS
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General Information
Symbol
Dmel\C3GMS
Species
D. melanogaster
Name
FlyBase ID
FBal0261152
Feature type
allele
Associated gene
Dmel\C3G
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
FLPase
(Shirinian et al., 2010)
Progenitor genotype
P{XP}d00064
(Shirinian et al., 2010)
PBac{RB}C3Ge03301
(Shirinian et al., 2010)
Cytology
Description

Generated by heat shock FLP-induced FRT-mediated recombination of PBac{RB}C3Ge03301 and P{XP}d00064, resulting in a 17.615kbp deletion of most of the C3G coding region.

(Shirinian et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:6,779,686..6,797,273 [-]
Comment:

Deletion of most of C3G resulting from FLP-catalyzed recombination using the FRT-containing transposons P{XP}d00064 and PBac{RB}C3Ge03301.

(Shirinian et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  cancer
      modeled by AlkUAS.cLa
      (Wolfstetter et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous flies are viable and fertile but have a reduced eclosion rate compared to wild type. Approximately 70% of C3GMS mutants hatch into larvae, with only 32% eclosing into adult flies.

      Adult flies have a shorter lifespan than controls, with a survival rate of 50% after 9 days.

      Ventral longitudinal muscle detachment, mistargeting and thinning is seen in 90% of 3rd instar larvae compared to 30% of controls. Muscle defects can be detected in second instar larvae but are not seen during the late embryonic stages.

      The sarcomeric muscle architecture is disorganised in homozygous larvae compared to wild type.

      TUNEL assays do not detect any difference between apoptosis levels in mutant larval muscles and controls.

      (Shirinian et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      C3GMS is partially rescued by Scer\GAL4Mef2.PR/C3GUAS.Tag:MYC

      (Shirinian et al., 2010)
      Comments

      Expression of C3GScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mef2.PR rescues 75% of the muscle defects seen in C3GMS homozygotes.

      (Shirinian et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      C3GMS
      (Wolfstetter et al., 2020)
      ΔC3GMS
      (Shirinian et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)