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General Information
Symbol
Dmel\dila81
Species
D. melanogaster
Name
FlyBase ID
FBal0262490
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Approximate endpoints of a ~2.0 kb deletion resulting from the imprecise excision of P{GT1}CG30001BG02674.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Caused by aberration
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{GT1}CG30001BG02674 resulting in a 2 kb deletion in which some of the CG30001 5'UTR and all/most of the DNA encoding the dila C-terminal coiled-coil domain is removed.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygotes reach the adult stage and these mutant flies show uncoordinated behaviour. They have a dramatically reduced climbing ability in bang tests compared with wild type controls. They are incapable of standing or righting themselves. They die very soon after eclosion.

Cilia of chordotonal neurons within Johnston's organ (in the adult antenna) show a range of ultrastructural defects in dila81 mutants. Some cilia are shortened; others are present but usually have a disorganised axonemal structure, often with missing microtubule doublets, or are occasionally grossly abnormal in structure or orientation. In addition, the doublets mostly lack their associated dynein arms. In contrast to the ciliary compartment, basal sections show no obvious defects in the ciliary rootlet or basal bodies and their associated fibrous structures. Likewise, no obvious defects are seen in the putative 'transition zone' beyond the basal bodies.

Chordotonal neuronal cilia are also missing or shortened in dila81 embryos and larvae.

dila81 male flies in which peripheral nervous system defects are substantially rescued by dilaScer\UAS.N.T:Zzzz\FLAG or dilaScer\UAS.C.T:Zzzz\FLAG expression show a very low level of fertility.

dila81 testes are of normal size and shape but few, if any, mature motile sperm are evident in 2-day old testes. No defects in meiotic cysts and a very low level of defects in onion-stage spermatocytes are seen. However, during the elongating stage, sperm nuclei show substantial disorganization: some nuclei are misorientated within the nuclear clusters and others are dispersed along the flagellar bundles. In addition, substantial disorganization of the flagellar bundles is observed and basal body markers are mislocalized. There are also individualization defects with many sperm tails sharing syncitial cytoplasm or lacking surrounding cytoplasmic membrane. Many flagella are separated from their mitochondrial derivatives and mixed axoneme polarities are observed. Despite these defects, flagellar axoneme structure appears intact.

Neuronal cell division is normal in dila81 embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

dila81;Cby1 double mutant flies are completely uncoordinated and show ultrastructural defects in chordotonal organ of the second antennal segment: cilia are mostly missing - the few remaining ones are severely disorganized and although both basal bodies (proximal and distal) are present, no transition zone is formed. The males are completely sterile, produce no mature sperm, their sperm cysts fail to elongate (but the overall size of their testes is not reduced) and show severe ultrastructural defects: Most of the axonemes are missing or damaged, the centrioles of late spermatocytes do not form a primary cilium-like structure, fail to dock to the plasma membrane, have an abnormal orientation in the cell, no ciliary cap is observed in these spermatocytes and premature axoneme elongation occurs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (2)