FlyBase Allele Report: Dmel\Tsc1[GL00012]

General Information

Symbol

Dmel\Tsc1^GL00012

Species

D. melanogaster

Name

FlyBase ID

FBal0262503

Feature type

allele

Associated gene

Dmel\Tsc1

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00012}

Also Known As

UAS-Tsc1 RNAi, Tsc1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00012}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00012

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

abnormal stress response | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

increased cell death | female limited | nutrition conditional, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Wei and Lilly, 2014)

Phenotype Manifest In

egg chamber | nutrition conditional, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Wei and Lilly, 2014)

female germline stem cell, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

Detailed Description

Statement

Reference

Expression of Tsc1^GL00012 in cardiac muscle under the control of Scer\GAL4^GMR88D05 has no effect on pupal eclosion rates.

Expression of Tsc1^GL00012 in skeletal muscle under the control of Scer\GAL4^how-24B has no effect on pupal eclosion rates.

(Hatfield et al., 2015)

When expressed with "MTD-GAL4" (which comprises Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{otu.T:Hsim\VP16}) in females that were starved for amino acids, then there is a marked increase in the number of degenerating (apoptotic) young egg chambers, compared to controls.

(Wei and Lilly, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012 is a suppressor of decreased cell growth rate phenotype of Wdr24¹

(Cai et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012 is a suppressor of ovary phenotype of Wdr24¹

(Cai et al., 2016)

Tsc1^GL00012, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of ovary phenotype of Akt^HMS00007, Scer\GAL4^{VP16.nanos.UTR}

(Wei and Lilly, 2014)

Scer\GAL4^nanos.PG/Tsc1^GL00012 is a suppressor of egg chamber phenotype of mio²/mio¹

(Wei et al., 2014)

Scer\GAL4^nanos.PG/Tsc1^GL00012 is a suppressor of egg chamber phenotype of Nup44A^Δ15

(Wei et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012 is a non-suppressor of autolysosome phenotype of Wdr24¹

(Cai et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tsc1^GL00012 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} significantly suppresses the ovary growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr24¹/Wdr24¹ flies.

(Cai et al., 2016)

Co-expression of Tsc1^GL00012 rescues the small ovary phenotype resulting from Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of Akt1^HMS00007.

(Wei and Lilly, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)