UAS regulatory sequences drive expression of a short inverted repeat.
Expression of ballGL00068 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in severe condensation defects (amorphous or undercondensed chromosomes with compromised attachment between sister chromatids) and failure to assemble spindle microtubules in mature oocytes and severe disruption of karyosome (compact body of condensed meiotic chromosome) formation during prophase I in stage 12 or 13 maturing oocytes.
Females heterozygous for ballGL00068 and MTD-Gal4 (consists of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16) crossed to ballGL00068 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.
Scer\GAL4VP16.mat.αTub67C, ballGL00068 has oocyte phenotype, suppressible | partially by Scer\GAL4VP16.mat.αTub67C/bafHMS00195
Scer\GAL4VP16.mat.αTub67C, ballGL00068 has chromosome | female | adult stage phenotype, suppressible | partially by Scer\GAL4VP16.mat.αTub67C/bafHMS00195
The severe condensation defects resulting in amorphous or undercondensed chromosomes in mature oocytes characteristic for flies expressing ballGL00068 RNAi under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 are significantly ameliorated by co-expression of bafHMS00195 RNAi.