FlyBase Allele Report: Dmel\Kdm5[GLV21071]

General Information

Symbol

Dmel\Kdm5^GLV21071

Species

D. melanogaster

Name

FlyBase ID

FBal0263052

Feature type

allele

Associated gene

Dmel\Kdm5

Associated Insertion(s)

Carried in Construct

P{TRiP.GLV21071}

Also Known As

UAS-KDM5^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GLV21071}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GLV21071

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of syndromic intellectual disability

CEA

(Hatch et al., 2021)

model of syndromic X-linked intellectual disability Claes-Jensen type

CEA

(Yheskel et al., 2024)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome, centromeric region | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

chromosome | adult stage | female, with Scer\GAL4^{VP16.nanos.UTR}

(Navarro-Costa et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Act5C.PI

(Belalcazar et al., 2021)

germarium | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

karyosome, with Scer\GAL4^{VP16.nanos.UTR}

(Navarro-Costa et al., 2016)

karyosome | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Navarro-Costa et al., 2016)

oocyte | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

ovary | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

synaptonemal complex | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

type Ib terminal bouton | decreased number | third instar larval stage, with Scer\GAL4^Act5C.PI

(Belalcazar et al., 2021)

type Ib terminal bouton | third instar larval stage, with Scer\GAL4^Act5C.PI

(Belalcazar et al., 2021)

Detailed Description

Statement

Reference

The expression of Kdm5^GLV21071 under the control of Scer\GAL4^Act5C.PI results in third instar larval muscles exhibiting altered NMJ morphology, with decreased number and increased size of type Ib synaptic boutons.

(Belalcazar et al., 2021)

Expression of lid^GLV21071 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in premature transcriptional reactivation as well as precocious and aberrant karyosome remodeling (the oocytes display early increase in chromatin openness and overly developed chromatin protrusions compared to controls) in prophase I-arrested oocytes (this however is not due to the incapability of the oocytes to enter transcriptional quiescence or to compact their chromosomes into karyosome in the first place). The metaphase I arrest of mature oocytes is not affected. Expression of lid^GLV21071 compromises meiotic completion as illustrated by defects in polar body integrity (scattered chromatin, abnormal chromosome condensation) and the eggs (though normal in size and dorsal-ventral patterning) largely fail to enter embryogenesis after fertilization resulting in strongly reduced female fertility.

(Navarro-Costa et al., 2016)

Expression of lid^GLV21071 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in female germlines results in sterility and abnormal morphology of the karyosome in most oocytes; a centromere clustering defect in the germaria wherein cohesion of sister chromosomes is maintained, but pairing of homologous pericentromeric regions of chromosomes 2 and 3 is disrupted; defects in the assembly and/or maintenance of the synaptonemal complex; defects in chromosome positioning and orientation in metaphase I oocytes; but does not cause significantly different numbers of DNA double stranded breaks in the germaria, as compared to controls.

(Zhaunova et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, non-enhanceable by Su(var)3-3^HMS00638, Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

NOT suppressed by

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, non-suppressible by Su(var)3-3^HMS00638, Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

Other

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR}, dhd^+t4.3, dhd^J5 has female semi-sterile phenotype

(Torres-Campana et al., 2022)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has karyosome | adult stage phenotype, non-enhanceable by Su(var)3-3^HMS00638, Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

NOT suppressed by

Statement

Reference

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has karyosome | adult stage phenotype, non-suppressible by Su(var)3-3^HMS00638, Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has karyosome | adult stage phenotype, non-suppressible by lok[+]/lok^p6

(Zhaunova et al., 2016)

Kdm5^GLV21071, Scer\GAL4^{VP16.nanos.UTR} has karyosome | adult stage phenotype, non-suppressible by p53^GL01220, Scer\GAL4^{VP16.nanos.UTR}

(Zhaunova et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Su(var)3-3^HMS00638 does not enhance or suppress the defective karyosome morphology seen in flies expressing lid^GLV21071 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

lok^p6/+ fails to suppress the abnormal karyosome morphology observed in females expressing lid^GLV21071 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Co-expression of p53^GL01220 fails to suppress the abnormal karyosome morphology observed in females expressing lid^GLV21071 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Zhaunova et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35706

y¹ sc^* v¹ sev²¹; P{TRiP.GLV21071}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Kdm5^GLV21071

lid^GLV21071

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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