FlyBase Allele Report: Dmel\cact[HMS00084]

General Information

Symbol

Dmel\cact^HMS00084

Species

D. melanogaster

Name

FlyBase ID

FBal0263089

Feature type

allele

Associated gene

Dmel\cact

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00084}

Also Known As

HMS00084, UAS-cact RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS00084}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00084

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mef2.PR

(Buffolo et al., 2015)

melanotic mass phenotype, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

Phenotype Manifest In

fat body, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

melanotic mass, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

Detailed Description

Statement

Reference

Expression of cact^HMS00084 under the control of Scer\GAL4^Mef2.PR (with UAS-Dicer2 to increase RNAi efficiency) results in a significant reduction in speed in third instar larvae.

(Buffolo et al., 2015)

Expression of cact^HMS00084 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in melanisation of the fat body.

(Zang et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

cact^HMS00084/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Suppressor of

Statement

Reference

cact^HMS00084/Scer\GAL4^r4 is a suppressor of abnormal immune response | adult stage phenotype of Scer\GAL4^r4, yki^UAS.cHa

(Liu et al., 2016)

Scer\GAL4^da.PU/cact^HMS00084 is a suppressor | partially of lethal phenotype of Scer\GAL4^da.PU, mad2^GD16427

(Liu et al., 2015)

NOT Suppressor of

Statement

Reference

cact^HMS00084/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

cact^HMS00084/Scer\GAL4^GMR.PF is a non-enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

NOT Suppressor of

Statement

Reference

cact^HMS00084/Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of cact^HMS00084 suppresses the reduced survival rates in response to Staphylococcus aureus (and the increase in bacterial load) seen in male flies with overexpression of yki^Scer\UAS.cHa driven in the fat body by Scer\GAL4^r4.

(Liu et al., 2016)

Under 30[o]C temperature conditions, the expression of mad2^GD16427 under the control of Scer\GAL4^da.PU induces lethality before adulthood, which is partially suppressed by the co-expression of cact^HMS00084.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34775