FlyBase Allele Report: Dmel\Sf3b5[HMS00097]

General Information

Symbol

Dmel\Sf3b5^HMS00097

Species

D. melanogaster

Name

FlyBase ID

FBal0263102

Feature type

allele

Associated gene

Dmel\Sf3b5

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00097}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS00097}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00097

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

female sterile, with Scer\GAL4^VP16.otu

(Yan and Perrimon, 2015)

lethal, with Scer\GAL4^nub-AC-62

(Yan and Perrimon, 2015)

lethal | female limited, with Scer\GAL4^dome-PG14

(Yan and Perrimon, 2015)

male semi-sterile, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

spermatogonium, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

spermatozoon, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

Detailed Description

Statement

Reference

Expressing Sf3b5^HMS00097 under the control of Scer\GAL4^Cg.PA leads to fragmented mitochondria in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Expression of CG11985^HMS00097 driven by Scer\GAL4^{bam.T:Hsim\VP16} leads to almost complete male sterility, along with defects in spermatogonial differentiation and absence of mature sperm.

(Wu et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34788

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00097}attP2

NIG-Fly

HMS00097

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00097}attP2

Notes on Origin

Discoverer

Comments

Supplemental Dataset_S01.

(Yan and Perrimon, 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG11985^HMS00097

Sf3b5^HMS00097

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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