FlyBase Allele Report: Dmel\rl[HMS00173]

General Information

Symbol

Dmel\rl^HMS00173

Species

D. melanogaster

Name

FlyBase ID

FBal0263168

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00173}

Also Known As

UAS-rl-RNAi, ERK RNAi, UAS-Erk RNAi, rl^RNAi, UAS-rl^RNAi, HMS00173

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS00173}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00173

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates prostate cancer

modeled by Ras85D^V12.UAS

(Rambur et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone, with Scer\GAL4^{Act5C.CoinFLP-FRT}

(Bosch et al., 2015)

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone, with Scer\GAL4^Act5C.PI

(Bosch et al., 2015)

decreased cell number | spermatogenesis, with Scer\GAL4^NP1624

(Zheng et al., 2021)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

increased cell death | spermatogenesis, with Scer\GAL4^NP1624

(Zheng et al., 2021)

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^NP1624

(Zheng et al., 2021)

long lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Slack et al., 2015)

long lived | RU486 conditional, with Scer\GAL4^Switch1.106

(Slack et al., 2015)

visible | adult stage, with Scer\GAL4^ptc-559.1

(Hall and Verheyen, 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Posterior dorsocentral bristle neurons expressing rl^HMS00173 under the control of Scer\GAL4^pnr-MD237 do not show obvious projection defects compared to controls.

(Urwyler et al., 2019)

Clones expressing rl^HMS00173 (using P{CoinFLP-GAL4} and Scer\FLP1^ey.PN to stochastically generate patches of cells expressing Scer\GAL4^{Act5C.CoinFLP-FRT}) are eliminated from the adult eye and overall eye size is reduced.

Expression of rl^HMS00173 using P{AyGAL4} and Scer\FLP1^ey.PN to generate clones in the eye expressing Scer\GAL4^Act5C.PI results in near-complete loss of the adult eye.

(Bosch et al., 2015)

Expressing rl^HMS00173 under the control of Scer\GAL4^ptc-559.1 results in loss of wing vein material in the anterior crossvein.

Expressing rl^HMS00173 under the control of either Scer\GAL4^dpp.blk1 or Scer\GAL4^ptc-559.1 has no effect on cell death in wing discs.

(Hall and Verheyen, 2015)

Females heterozygous for rl^HMS00173 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to rl^HMS00173 males results in: 0% hatching; egg/embryonic phenotype: fusion of denticles in ventral midline; maternal effect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

rl^HMS00173/Scer\GAL4^Tub.PU is a suppressor | partially of increased size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

rl^HMS00173, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of increased size | oogenesis phenotype of Ras85D^V12.cZa.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Zhang et al., 2024)

rl^HMS00173/Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, aux^NIG.1107R

(Zhao et al., 2022)

NOT Suppressor of

Statement

Reference

rl^HMS00173/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of decreased cell number | recessive | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^org-1.HN39, rl^HMS00173 has adult heart ventral longitudinal muscle cell | P-stage phenotype, suppressible | partially by Scer\GAL4^org-1.HN39/yki^{S168A.UAS.EGFP,Tag:HA}

(Rose et al., 2022)

Suppressor of

Statement

Reference

rl^HMS00173/Scer\GAL4^Tub.PU is a suppressor | partially of follicle cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

rl^HMS00173, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of germarium phenotype of Ras85D^V12.cZa.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Zhang et al., 2024)

rl^HMS00173, Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of spectrosome | oogenesis phenotype of Ras85D^V12.cZa.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Zhang et al., 2024)

rl^HMS00173/Scer\GAL4^NP5130 is a suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^NP5130, aux^NIG.1107R

(Zhao et al., 2022)

rl^HMS00173/Scer\GAL4^tin.CΔ4 is a suppressor | partially of adult heart phenotype of Scer\GAL4^tin.CΔ4, yki^{S168A.UAS.Tag:V5}

(Yu et al., 2015)

NOT Suppressor of

Statement

Reference

rl^HMS00173/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of germline cell | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Dsor1^HMS00145 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not suppress the decreased primordial germ cells in rl^HMS00173 homozygous early embryos (i.e. mitotic cycles 12/13).

(Pae et al., 2017)

Expression of rl^HMS00173 partially suppresses the cardiac hypertrophy seen when yki^{S168A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^tin.CΔ4.

(Yu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34855