FlyBase Allele Report: Dmel\SCAR[HMS01536]

General Information

Symbol

Dmel\SCAR^HMS01536

Species

D. melanogaster

Name

FlyBase ID

FBal0263439

Feature type

allele

Associated gene

Dmel\SCAR

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01536}

Also Known As

UAS-SCAR RNAi, UAS.SCAR-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01536}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01536

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage, with Scer\GAL4^hh-Gal4

(Bischoff et al., 2013)

Phenotype Manifest In

cortical actin cytoskeleton | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

cytoneme | larval stage, with Scer\GAL4^hh-Gal4

(Bischoff et al., 2013)

egg | maternal effect, with Scer\GAL4^GR1

(Squarr et al., 2016)

egg | maternal effect, with Scer\GAL4^tj.PU

(Squarr et al., 2016)

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

embryonic/larval lymph gland, with Scer\GAL4^kn.PU

(Fuwa et al., 2015)

embryonic/larval plasmatocyte | increased number, with Scer\GAL4^kn.PU

(Fuwa et al., 2015)

female germline ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4^elav.PU

(Kannan et al., 2017)

lymph gland posterior signaling center cell, with Scer\GAL4^kn.PU

(Fuwa et al., 2015)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wing disc posterior compartment, with Scer\GAL4^hh-Gal4

(Bischoff et al., 2013)

Detailed Description

Statement

Reference

Expression of SCAR^HMS01536 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to premature ooplasmic streaming in stage 7 oocytes, actin defects at the oocyte cortex and at the ring canals in stage 7-9 egg chambers when compared to controls.

(Verboon et al., 2018)

Expression of SCAR^HMS01536 under the control of Scer\GAL4^elav.PU induces stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 (observed in ~25% of examined hemisegments).

(Kannan et al., 2017)

Expression of SCAR^HMS01536 under the control of Scer\GAL4^GR1, or under the control of Scer\GAL4^tj.PU along with a Dicer-2 transgene to increase RNAi efficiency, results in females laying abnormally round eggs, as compared to controls.

(Squarr et al., 2016)

Expression of SCAR^HMS01536 in the lymph gland under the control of Scer\GAL4^kn.PU results in a reduction in the number of hematopoietic stem cells and an increase in the number of mature plasmatocytes.

(Fuwa et al., 2015)

The expression of SCAR^HMS01536 under the control of Scer\GAL4^hh-Gal4 leads to a decreased maximum cytoneme length at the A-P wing disc compartment border, as compared to controls.

(Bischoff et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C} has cortical actin cytoskeleton | oogenesis phenotype, non-enhanceable by wash^Δ185/wash^Δ185

(Verboon et al., 2018)