Allele Dmel\Antp^{Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\Antp^{Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0263471
Feature type	allele	Associated gene	Dmel\Antp

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Papadopoulos et al., 2011) in vitro construct - coding region fusion (Papadopoulos et al., 2011) in vitro construct - regulatory fusion (Papadopoulos et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a synthetic version of Antp which lacks the N-terminal domain. The protein consists of a YPWM motif which has been mutated to AAAA and the homeodomain, followed by a truncated C-terminal domain (lacking the terminal 14 residues). The naturally occurring long linker (8 amino acid residues) is present between the mutated AAAA motif and the homeodomain. A T:Avic\GFP[EGFP] tag has been fused to the C-terminal end of the protein. (Papadopoulos et al., 2011)
Carried in construct	P{UAS-Antp.Synth.AAAA.LL.eGFP} (Papadopoulos et al., 2011)
Tagged with	T:Avic\GFP^EGFP (Papadopoulos et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^Dll.PU (Papadopoulos et al., 2011)
Phenotype Manifest In
	arista, with Scer\GAL4^Dll.PU (Papadopoulos et al., 2011) mouth hooks, with Scer\GAL4^nullo.PG (Papadopoulos et al., 2011) third segment of antenna, with Scer\GAL4^Dll.PU (Papadopoulos et al., 2011)
Detailed Description
	Statement Reference Expression of Antp[Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[nullo.PG] does not result in transformation of head to mesothorax and the prothoracic beard is present. Mild head defects such as abnormal development of the mouth hook are seen. Expression of Antp[Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Dll.PU] results in no transformation of the antenna or only a mild thickening of the arista with a few ectopic leg bristles on the third antennal segment. (Papadopoulos et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Antp^{Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Papadopoulos et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 11959--11964 Functional synthetic Antennapedia genes and the dual roles of YPWM motif and linker size in transcriptional activation and repression. [FBrf0214342]

Allele Dmel\AntpSynth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP

Allele Dmel\Antp^{Synth.AAAA.LL.Scer\UAS.T:Avic\GFP-EGFP}