Allele Dmel\sqh^DD.Scer\UAS

General Information
Symbol	Dmel\sqh^DD.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0263515
Feature type	allele	Associated gene	Dmel\sqh

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Mitonaka et al., 2007) in vitro construct - regulatory fusion (Jiang et al., 2011, Mitonaka et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a phosphomimetic (activated) form of sqh. (Jiang et al., 2011) Scer\UAS regulatory sequences drive expression of an activated form of sqh carrying amino acid substitutions T20E and S21E to mimic the constitutive phosphorylated form. (Mitonaka et al., 2007)
Carried in construct	P{UAS-sqh.DD} (Mitonaka et al., 2007, Tang et al., 2011, Jiang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	eye disc, with Scer\GAL4^ey.PU (Jiang et al., 2011) eye disc \| somatic clone, with Scer\GAL4^Act.PU (Jiang et al., 2011) wing disc \| somatic clone \| third instar larval stage, with Scer\GAL4^Act5C.PI (Mitonaka et al., 2007) wing disc \| third instar larval stage, with Scer\GAL4^Bx-MS1096 (Mitonaka et al., 2007) wing disc \| third instar larval stage, with Scer\GAL4^ptc-559.1 (Mitonaka et al., 2007)
Detailed Description
	Statement Reference Cells from clones in the eye disc expressing sqh[DD.Scer\UAS] under the control of Scer\GAL4[Act.PU] become mislocalised in the optic stalk. Cells from the eye disc expressing sqh[DD.Scer\UAS] under the control of Scer\GAL4[ey.PU] become mislocalised in the optic stalk. (Jiang et al., 2011) Wing discs overexpressing sqh[DD.Scer\UAS] under the control of Scer\GAL4[Bx-MS1096] are multi-layered and show malformed disc shapes. In contrast to the columnar wild-type cells, wing disc cells in clones overexpressing sqh[DD.Scer\UAS] driven by Scer\GAL4[Act5C.PI] shrink basally and show abnormal apical-basal polarity. In contrast to wild-type controls, cells overexpressing sqh[DD.Scer\UAS] under the control of Scer\GAL4[ptc-559.1] appear to migrate out from the Scer\GAL4[ptc-559.1]-expression domain. (Mitonaka et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference sqh^DD.Scer\UAS/Scer\GAL4^Act5C.PP is a suppressor of autophagic vacuole \| somatic clone \| calorie restriction conditional phenotype of Scer\GAL4^Act5C.PP, sqa^{dsRNA.Scer\UAS} (Tang et al., 2011) sqh^DD.Scer\UAS/Scer\GAL4^Act5C.PP is a suppressor of fat body \| somatic clone \| calorie restriction conditional phenotype of Scer\GAL4^Act5C.PP, sqa^{dsRNA.Scer\UAS} (Tang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of sqh[DD.Scer\UAS] with sqa[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act5C.PP] suppresses the sqa[dsRNA.Scer\UAS]-induced autophagic defects in larval fat body cells. (Tang et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Sqh^D20D21 (Mitonaka et al., 2007) sqh^DD.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Jiang et al., 2011, Oncogene 30(29): 3248--3260 Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling. [FBrf0214488] Tang et al., 2011, EMBO J. 30(4): 636--651 Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. [FBrf0213047] Mitonaka et al., 2007, Dev. Biol. 309(1): 78--86 Essential roles of myosin phosphatase in the maintenance of epithelial cell integrity of Drosophila imaginal disc cells. [FBrf0200468]

Allele Dmel\sqhDD.Scer\UAS

Allele Dmel\sqh^DD.Scer\UAS