Allele Dmel\Vav^{Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\Vav^{Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0263651
Feature type	allele	Associated gene	Dmel\Vav

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Couceiro et al., 2005) in vitro construct - regulatory fusion (Couceiro et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 3.0kb full length vav coding sequence is expressed under the control of Scer\UAS regulatory sequences and tagged at the C-terminal with T:Ivir\HA1. Two alterations to the sequence are present, causing M53T and a silent codon change in codon 313. (Couceiro et al., 2005) Amino acid replacement: M53T. (Couceiro et al., 2005)
Carried in construct	P{UAS-vav.HA} (Couceiro et al., 2005, Malartre et al., 2010)
Tagged with	T:Ivir\HA1 (Couceiro et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Embryos expressing vav[Scer\UAS.T:Ivir\HA1] in the epidermis using the Scer\GAL4[69B] driver are indistinguishable from wild type. Embryos expressing vav[Scer\UAS.T:Ivir\HA1] in the mesoderm using the Scer\GAL4[how-24B] driver do not display any obvious muscle defects. Embryos expressing vav[Scer\UAS.T:Ivir\HA1] under the control of the nervous system driver Scer\GAL4[elav.PLu] are indistinguishable from wild type. Caudal visceral cell (CVM) migration is comparable to wild type when vav[Scer\UAS.T:Ivir\HA1] is expressed in embryos under the control of the Scer\GAL4[447.2] driver. Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of the tracheal system driver Scer\GAL4[btl.PS] has no effect on tracheal development. (Couceiro et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^arm.PS/Vav^{Scer\UAS.T:Ivir\HA1} rescues Vav² (Malartre et al., 2010) Scer\GAL4^arm.PS/Vav^{Scer\UAS.T:Ivir\HA1} rescues Vav³ (Malartre et al., 2010) Vav^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^αTub84B.PL rescues Vav² (Malartre et al., 2010) Vav^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^αTub84B.PL rescues Vav³ (Malartre et al., 2010)
Partially rescues	Scer\GAL4^arm.PS/Vav^{Scer\UAS.T:Ivir\HA1} partially rescues Vav² (Malartre et al., 2010) Scer\GAL4^arm.PS/Vav^{Scer\UAS.T:Ivir\HA1} partially rescues Vav³ (Malartre et al., 2010)
Fails to rescue	Vav^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^GMR.PU fails to rescue Vav¹ (Malartre et al., 2010)
Comments	Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of either the Scer\GAL4[arm.PS] or the Scer\GAL4[αTub84B.PL] driver rescues the semi-lethality seen in vav[2] male homozygotes. Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of either the Scer\GAL4[arm.PS] or the Scer\GAL4[αTub84B.PL] driver rescues the semi-lethality seen in vav[3] male homozygotes. Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[arm.PS] partially rescues the midline crossing phenotype seen in vav[2] homozygotes. Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[arm.PS] partially rescues the midline crossing phenotype seen in vav[3] homozygotes. Expression of vav[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[GMR.PU] fails to rescue the photoreceptor targeting phenotype seen in vav[1] homozygotes. (Malartre et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	vav^{Scer\UAS.T:Ivir\HA1} Vav^{Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Malartre et al., 2010, J. Neurosci. 30(6): 2257--2267 The Guanine Exchange Factor vav Controls Axon Growth and Guidance during Drosophila Development. [FBrf0209937] Couceiro et al., 2005, Exp. Cell Res. 308(2): 364--380 Phylogenetic conservation of the regulatory and functional properties of the Vav oncoprotein family. [FBrf0187549]

Allele Dmel\VavScer\UAS.T:Ivir\HA1

Allele Dmel\Vav^{Scer\UAS.T:Ivir\HA1}