FB2025_01 , released February 20, 2025
Allele: Dmel\VavΔ1-207.UAS.Tag:HA
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General Information
Symbol
Dmel\VavΔ1-207.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0263652
Feature type
allele
Associated gene
Dmel\Vav
Associated Insertion(s)
Carried in Construct
P{UAS-Vav.Δ1-207.HA}
Key Links
Transgenic product class
inframe_deletion
(Couceiro et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Couceiro et al., 2005)
Mutagen
in vitro construct
(Couceiro et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-Vav.Δ1-207.HA}
Cytology
Description

A constitutively active form of Vav, comprising a deletion of the N-terminus (amino acids 1-207), is expressed under the control of UASt regulatory sequences and tagged at the C-terminal end with Tag:HA. A silent codon change is present in codon 313.

(Couceiro et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vav
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of constitutively active vavΔ1-207.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU results in severe disruption to adult eye morphology.

      (Malartre et al., 2010)

      Expression of vavΔ1-207.Scer\UAS.T:Ivir\HA1 in the epidermis of embryos from stage 9 onwards using the Scer\GAL469B driver results in dorsal closure failure. The lateral epidermal cells are disorganised and fail to elongate.

      Expression of vavΔ1-207.Scer\UAS.T:Ivir\HA1 in the embryonic mesoderm using the Scer\GAL4how-24B driver results in inhibition of myoblast fusion.

      Expression of vavΔ1-207.Scer\UAS.T:Ivir\HA1 under the control of the nervous system driver Scer\GAL4elav.PLu results in embryos that display axon guidance defects such as misrouting of longitudinal axons across the midline and gaps between segments. The axons appear thicker than in wild type embryos. Axons also fail to extend between the dorsal and lateral clusters of the peripheral nervous system.

      When vavΔ1-207.Scer\UAS.T:Ivir\HA1 is expressed in the caudal visceral mesoderm (CVM) cells under the control of Scer\GAL4447.2 they fail to migrate towards the anterior pole of the embryo. The CVM cells remain viable at the posterior end of the embryo, indicating that cell survival is unaffected.

      Expression of vavΔ1-207.Scer\UAS.T:Ivir\HA1 under the control of the tracheal system driver Scer\GAL4btl.PS results in a variety of defects in the embryo including severe truncation of the tracheal dorsal trunk, misguided dorsal branching and limited terminal branching differentiation.

      (Couceiro et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, VavΔ1-207.UAS.Tag:HA has visible phenotype, non-suppressible by Rac2Δ/Rac2[+]

      (Malartre et al., 2010)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, VavΔ1-207.UAS.Tag:HA has eye phenotype, suppressible by Rac2Δ/Rac2Δ

      (Malartre et al., 2010)

      Scer\GAL4GMR.PU, VavΔ1-207.UAS.Tag:HA has eye phenotype, suppressible by MtlΔ/MtlΔ

      (Malartre et al., 2010)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, VavΔ1-207.UAS.Tag:HA has eye phenotype, non-suppressible by Rac2Δ/Rac2[+]

      (Malartre et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The eye phenotype seen when vavΔ1-207.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU is suppressed in a homozygous Rac2Δ background. The phenotype is not suppressed in a Rac2Δ/+ background.

      The eye phenotype seen when vavΔ1-207.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU is suppressed in a homozygous MtlΔ mutant background.

      (Malartre et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      VavΔ1-207.Scer\UAS.T:Ivir\HA1
      VavΔ1-207.UAS.Tag:HA
      vavΔ1-207.Scer\UAS.T:Ivir\HA1
      Name Synonyms
      Secondary FlyBase IDs
        References (4)