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General Information
Symbol
Dmel\pblΔN-term.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0263872
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated form of pbl-RA cDNA (amino acids 386-853) that lacks the N-terminal regulatory domains and is tagged at the C-terminal end with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4e22c-mediated expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 results in 100% embryonic lethality and epidermal fragmentation.

Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the eye under the control of the Scer\GAL4GMR.PU driver results in pupal lethality. At 18[o]C the lethality occurs at the pharate adult stage. Pupal flies exhibit a strong eye phenotype.

Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the mesoderm of stage 8 embryos under the control of Scer\GAL4twi.2PE and Scer\GAL4Mef2.PR drivers blocks mesoderm invagination and the cells fail to undergo cytokinesis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has lethal | pharate adult stage | temperature conditional phenotype, suppressible by Rho1k07236/Rho1[+]

NOT suppressed by
Statement
Reference

Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has lethal | pharate adult stage | temperature conditional phenotype, non-suppressible by Rac2Δ/Mtl[+]/Rac1J10/MtlΔ/Rac2[+]/Rac1[+]

Other
Statement
Reference

Rho1k07236/Rho1[+], Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has visible | adult stage | temperature conditional phenotype

Phenotype Manifest In
Other
Statement
Reference

Rho1k07236/Rho1[+], Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has eye | adult stage | temperature conditional phenotype

Additional Comments
Genetic Interactions
Statement
Reference

One copy of Rho1k07236 suppresses the lethality seen when pblΔN-term.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Adult flies exhibit a strong eye phenotype.

One copy of each of Rac1J10, Rac2Δ and MtlΔ fails to suppress the lethality seen when pblΔN-term.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the mesoderm under the control of Scer\GAL4twi.2PE fails to rescue the mesodermal migration defects seen in pbl3 homozygous mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pblΔN-term.Scer\UAS.T:Ivir\HA1
pblΔN-term.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (2)