Allele Dmel\pbl^{DH-PH.Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\pbl^{DH-PH.Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0263873
Feature type	allele	Associated gene	Dmel\pbl

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (van Impel et al., 2009) in vitro construct - regulatory fusion (van Impel et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the DH and PH domains of pbl-RA (amino acids 386-775) tagged at the C-terminal end with T:Ivir\HA1. (van Impel et al., 2009)
Carried in construct	P{UAS-pbl.DH-PH.HA} (van Impel et al., 2009)
Tagged with	T:Ivir\HA1 (van Impel et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	cell migration defective \| embryonic stage 8, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.2PE (van Impel et al., 2009) visible \| adult stage, with Scer\GAL4^GMR.PU (van Impel et al., 2009)
Phenotype Manifest In
	eye \| adult stage, with Scer\GAL4^GMR.PU (van Impel et al., 2009) mesoderm \| embryonic stage 8, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.2PE (van Impel et al., 2009)
Detailed Description
	Statement Reference Expression of pbl[DH-PH.Scer\UAS.T:Ivir\HA1] in the mesoderm of stage 8 embryos under the control of the Scer\GAL4[twi.2PE] and Scer\GAL4[Mef2.PR] drivers results in defects in mesoderm migration. No defects in cytokinesis are observed. Expression of pbl[DH-PH.Scer\UAS.T:Ivir\HA1] in the eye under the control of the Scer\GAL4[GMR.PU] driver results in a rough eye phenotype with reduced eye size and highly abnormal ommatidial structures. (van Impel et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, enhanceable by Rac1^Scer\UAS.cLa (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, enhanceable by Rac2^EP3118 (van Impel et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, suppressible by Rac2^Δ/Mtl[+]/Rac1^J10/Mtl^Δ/Rac2[+]/Rac1[+] (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, suppressible by Rho1^k07236/Rho1[+] (van Impel et al., 2009)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, non-suppressible by Cdc42[+]/Cdc42⁴ (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, non-suppressible by RhoL^N25.Scer\UAS (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has visible \| adult stage phenotype, non-suppressible by RhoL^V20.Scer\UAS (van Impel et al., 2009)
Other
Statement Reference Rac1^Scer\UAS.cLa, Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has partially lethal - majority die \| pharate adult stage phenotype (van Impel et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, enhanceable by Rac1^Scer\UAS.cLa (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, enhanceable by Rac2^EP3118 (van Impel et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, suppressible by Rac2^Δ/Mtl[+]/Rac1^J10/Mtl^Δ/Rac2[+]/Rac1[+] (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, suppressible by Rho1^k07236/Rho1[+] (van Impel et al., 2009)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, non-suppressible by Cdc42[+]/Cdc42⁴ (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, non-suppressible by RhoL^N25.Scer\UAS (van Impel et al., 2009) Scer\GAL4^GMR.PU, pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} has eye \| adult stage phenotype, non-suppressible by RhoL^V20.Scer\UAS (van Impel et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference One copy of Rho1[k07236] suppresses the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. Co-expression of RhoL[V20.Scer\UAS] does not suppress the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. Co-expression of RhoL[N25.Scer\UAS] does not suppress the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. One copy of Cdc42[4] fails to suppress the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. One copy of each of Rac1[J10], Rac2[Δ] and Mtl[Δ] strongly suppresses the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. Co-expression of Rac1[Scer\UAS.cLa] strongly enhances the eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. Most flies die as pharate adults but escapers fail to develop any eye structures. Co-expression of Rac2[EP3118] strongly enhances the rough eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. (van Impel et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} is partially rescued by pbl³/Scer\GAL4^GMR.PU (van Impel et al., 2009)
Rescues	Scer\GAL4^twi.2PE/pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} rescues pbl³ (van Impel et al., 2009)
Comments	Expression of pbl[DH-PH.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[twi.2PE] rescues the mesodermal migration defects seen in pbl[3] homozygous mutants. A pbl[3]/+ background partially rescues the eye phenotype seen when pbl[DH-PH.Scer\UAS.T:Ivir\HA1] is expressed under the control of Scer\GAL4[GMR.PU]. (van Impel et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pbl^{DH-PH.Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	van Impel et al., 2009, Development 136(5): 813--822 Regulation of the Rac GTPase pathway by the multifunctional Rho GEF Pebble is essential for mesoderm migration in the Drosophila gastrula. [FBrf0206776]

Allele Dmel\pblDH-PH.Scer\UAS.T:Ivir\HA1

Allele Dmel\pbl^{DH-PH.Scer\UAS.T:Ivir\HA1}