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General Information
Symbol
Dmel\pblDH-PH.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0263873
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the DH and PH domains of pbl-RA (amino acids 386-775) tagged at the C-terminal end with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4e22c-mediated expression of pblDH-PH.Scer\UAS.T:Ivir\HA1 results in 57.3% embryonic lethality with 49% of embryos showing some loss of naked cuticle on the ventral side.

Expression of pblDH-PH.Scer\UAS.T:Ivir\HA1 in the mesoderm of stage 8 embryos under the control of the Scer\GAL4twi.2PE and Scer\GAL4Mef2.PR drivers results in defects in mesoderm migration. No defects in cytokinesis are observed.

Expression of pblDH-PH.Scer\UAS.T:Ivir\HA1 in the eye under the control of the Scer\GAL4GMR.PU driver results in a rough eye phenotype with reduced eye size and highly abnormal ommatidial structures.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4GMR.PU, pblDH-PH.UAS.Tag:HA has eye | adult stage phenotype, suppressible by Rac2Δ/Mtl[+]/Rac1J10/MtlΔ/Rac2[+]/Rac1[+]

NOT suppressed by
Statement
Reference
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of pblDH-PH.Scer\UAS.T:Ivir\HA1 partially suppresses the excess naked cuticle phenotype seen in Scer\GAL4arm.PS armS10.Scer\UAS.T:Hsap\MYC embryos.

One copy of Rho1k07236 suppresses the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Co-expression of RhoLV20.Scer\UAS does not suppress the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Co-expression of RhoLN25.Scer\UAS does not suppress the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

One copy of Cdc424 fails to suppress the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

One copy of each of Rac1J10, Rac2Δ and MtlΔ strongly suppresses the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Co-expression of Rac1Scer\UAS.cLa strongly enhances the eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Most flies die as pharate adults but escapers fail to develop any eye structures.

Co-expression of Rac2EP3118 strongly enhances the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of pblDH-PH.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4twi.2PE rescues the mesodermal migration defects seen in pbl3 homozygous mutants.

A pbl3/+ background partially rescues the eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pblDH-PH.Scer\UAS.T:Ivir\HA1
pblDH-PH.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (2)