Allele Dmel\Trc8^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Trc8^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0263918
Feature type	allele	Associated gene	Dmel\Trc8

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Gemmill et al., 2002) in vitro construct - regulatory fusion (Gemmill et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS drives expression of the Trc8 coding region, beginning 19 bp prior to the AUG, and ending with a T:Hsap\MYC tag and an in-frame stop codon. (Gemmill et al., 2002)
Carried in construct	P{UAS-Trc8.MYC} (Gemmill et al., 2005, Gemmill et al., 2002)
Tagged with	T:Hsap\MYC (Gemmill et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act.PU (Gemmill et al., 2002) lethal \| first instar larval stage, with Scer\GAL4^Act.PU (Gemmill et al., 2005) size defective, with Scer\GAL4^ap-md544 (Gemmill et al., 2002) size defective, with Scer\GAL4^Bx-MS1096 (Gemmill et al., 2005, Gemmill et al., 2002) size defective, with Scer\GAL4^en.PU (Gemmill et al., 2005)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PU (Gemmill et al., 2002) wing, with Scer\GAL4^ap-md544 (Gemmill et al., 2002) wing, with Scer\GAL4^Bx-MS1096 (Gemmill et al., 2005, Gemmill et al., 2002) wing, with Scer\GAL4^en.PU (Gemmill et al., 2005)
Detailed Description
	Statement Reference Scer\GAL4[Bx-MS1096], Trc8[Scer\UAS.T:Hsap\MYC] wings are small and deformed. Male wings show a more severe effect than females (probably because of dosage compensation resulting from Scer\GAL4[Bx-MS1096] being located on the X chromosome). Scer\GAL4[en.PU], Trc8[Scer\UAS.T:Hsap\MYC] wings are growth inhibited in the posterior compartment, showing an anterior/posterior ratio of 2.36 compared to 1.03 for wild type. (Gemmill et al., 2005) Expression of Trc8[Scer\UAS.T:Hsap\MYC] using Scer\GAL4[Act.PU] is lethal, while expression using Scer\GAL4[sim.PS] or Scer\GAL4[prd.RG1] has no obvious effects. Expression of Trc8[Scer\UAS.T:Hsap\MYC] using Scer\GAL4[GMR.PU] results in loss of pigment and photoreceptors. Expression of Trc8[Scer\UAS.T:Hsap\MYC] using Scer\GAL4[Bx-MS1096] or Scer\GAL4[ap-md544] results in small, curled wings, compatible with preferential growth inhibition in the dorsal compartment. Using Scer\GAL4[Bx-MS1096], overall wing length and width of the wing is reduced by approximately 20%. These smaller wings result largely from there being fewer cells. (Gemmill et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, suppressible by CSN5[+]/CSN5^quo-3 (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, suppressible by CSN5^null/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, suppressible by Df(3R)hh/+ (Gemmill et al., 2005) Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, suppressible \| partially by CSN5[+]/CSN5^quo-3 (Gemmill et al., 2005) Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, suppressible \| partially by CSN5^null/CSN5[+] (Gemmill et al., 2005)
NOT suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, non-suppressible by CSN4[+]/CSN4^null (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, non-suppressible by CSN5^quo-1/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, non-suppressible by CSN5^quo-2/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, non-suppressible by Rpn8^k08003/Mov34[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has size defective phenotype, non-suppressible by Rpn11^BG01694/Rpn11[+] (Gemmill et al., 2005)
Other
Statement Reference CSN5^quo-1/CSN5[+], Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has lethal phenotype (Gemmill et al., 2005) CSN5^quo-2/CSN5[+], Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has lethal phenotype (Gemmill et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, suppressible by CSN5[+]/CSN5^quo-3 (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, suppressible by CSN5^null/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, suppressible by Df(3R)hh/+ (Gemmill et al., 2005) Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, suppressible \| partially by CSN5[+]/CSN5^quo-3 (Gemmill et al., 2005) Scer\GAL4^en.PU, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, suppressible \| partially by CSN5^null/CSN5[+] (Gemmill et al., 2005)
NOT suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, non-suppressible by CSN4[+]/CSN4^null (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, non-suppressible by CSN5^quo-1/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, non-suppressible by CSN5^quo-2/CSN5[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, non-suppressible by Rpn8^k08003/Mov34[+] (Gemmill et al., 2005) Scer\GAL4^Bx-MS1096, Trc8^{Scer\UAS.T:Hsap\MYC} has wing phenotype, non-suppressible by Rpn11^BG01694/Rpn11[+] (Gemmill et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference CSN5[null]/+ or CSN5[quo-3]/+ or Df(3R)hh/+ (Df(3R)hh uncovers CSN6) restores normal appearing growth to Scer\GAL4[Bx-MS1096], Trc8[Scer\UAS.T:Hsap\MYC] female wings and substantially increases wing size in male wings. CSN5[null]/+ or CSN5[quo-3]/+ partially restores the anterior/posterior size ratio of Scer\GAL4[en.PU], Trc8[Scer\UAS.T:Hsap\MYC] wings towards wild type. (Gemmill et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Trc8^{Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Gemmill et al., 2005, Oncogene 24(21): 3503--3511 Growth suppression induced by the TRC8 hereditary kidney cancer gene is dependent upon JAB1/CSN5. [FBrf0206286] Gemmill et al., 2002, Oncogene 21(22): 3507--3516 The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway. [FBrf0206144]

Allele Dmel\Trc8Scer\UAS.T:Hsap\MYC

Allele Dmel\Trc8^{Scer\UAS.T:Hsap\MYC}