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Allele Dmel\Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Pk92BΔN.Scer\UAS.T:Zzzz\FLAG	Species	D. melanogaster
Name		FlyBase ID	FBal0263920
Feature type	allele	Associated gene	Dmel\Pk92B
Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - deletion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms adult cuticle
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Sekine et al., 2011) in vitro construct - deletion (Sekine et al., 2011) in vitro construct - regulatory fusion (Sekine et al., 2011)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS drives expression of a cDNA encoding Pk92B that lacks the N-terminal 558 amino acids, with an N-terminal T:Zzzz\FLAG tag. This acts as a constitutively active form. (Sekine et al., 2011)
Carried in construct	P{UAS-Pk92B.ΔN} (Sekine et al., 2011, Sekine et al., 2012)
Tagged with	T:Zzzz\FLAG (Sekine et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4pnr-MD237 (Sekine et al., 2011, Sekine et al., 2012) body color defective | heat sensitive, with Scer\GAL4hs.PU (Sekine et al., 2011)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4pnr-MD237 (Sekine et al., 2012) adult thorax, with Scer\GAL4pnr-MD237 (Sekine et al., 2011) adult thorax | heat sensitive, with Scer\GAL4hs.PU (Sekine et al., 2011) wing | heat sensitive, with Scer\GAL4hs.PU (Sekine et al., 2011)
Detailed Description
	Statement Reference Expression of Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] results in an increase in melanisation of the adult thorax cuticle in the domain of Scer\GAL4[pnr-MD237] expression. (Sekine et al., 2012) Flies expressing Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] exhibit black pigmentation in the thorax cuticle. Ubiquitous expression of Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG] using Scer\GAL4[hs.PU], induced by a 1 hour heat shock during the pupal stage, results in black pigmentation only in the lower medial region of the thorax cuticle, together with a defect in wing expansion. (Sekine et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible | partially by Hr38dsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible | partially by Mkk4NIG.9738R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible | partially by p38cKK103439, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible | partially by y1 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by DdcNIG.10697R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by eScer\UAS.cWa, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by hepNIG.4153R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by licGD7546, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by Mpk2DN.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by Mpk2NIG.5475R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by p38bNIG.7393R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, suppressible by pledsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011)
NOT suppressed by
Statement Reference Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, non-suppressible by hep1 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has body color defective phenotype, non-suppressible by slimdsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2012)
Phenotype Manifest In
Suppressed by
Statement Reference Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible | partially by Hr38dsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible | partially by Mkk4NIG.9738R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible | partially by p38cKK103439, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible | partially by y1 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by DdcNIG.10697R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by eScer\UAS.cWa, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by hepNIG.4153R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by licGD7546, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by Mpk2DN.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by Mpk2NIG.5475R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by p38bNIG.7393R, Scer\GAL4pnr-MD237 (Sekine et al., 2011) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, suppressible by pledsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2011)
NOT suppressed by
Statement Reference Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult cuticle phenotype, non-suppressible by slimdsRNA.Scer\UAS, Scer\GAL4pnr-MD237 (Sekine et al., 2012) Pk92BΔN.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4pnr-MD237 has adult thorax phenotype, non-suppressible by hep1 (Sekine et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of slim[dsRNA.Scer\UAS] has no effect on the increase in melanisation of the adult thorax cuticle seen in flies expressing Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237]. (Sekine et al., 2012) Co-expression of Mpk2[DN.Scer\UAS], Mpk2[NIG.5475R], p38b[NIG.7393R], or lic[GD7546] abolishes the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. Co-expression of p38c[KK103439] has only a marginal suppressive effect. Co-expression of Mkk4[NIG.9738R] attenuates the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. Co-expression of hep[NIG.4153R] or hemizygosity for hep[1] does not suppress the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. Co-expression of e[Scer\UAS.cWa] abolishes the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. In a y[1] hemizygous background, pigmentation in the thorax of Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies is brown rather than black, particularly in the scutellum. Co-expression of either ple[dsRNA.Scer\UAS] or Ddc[NIG.10697R] abolishes the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. Co-expression of Hr38[dsRNA.Scer\UAS] reduces the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies at 18[o]C. (Sekine et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Pk92BΔN.Scer\UAS.T:Zzzz\FLAG is rescued by Scer\GAL4pnr-MD237/Pk92BdsRNA.Scer\UAS (Sekine et al., 2011)
Comments	Co-expression of Pk92B[dsRNA.Scer\UAS] suppresses the pigmentation defect in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. (Sekine et al., 2011)
Stocks ( 0 )
Notes on Origin
Discoverer
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Pk92BΔN.Scer\UAS.T:Zzzz\FLAG
Name Synonym
Secondary FlyBase IDs
References ( 2 )
Research paper	Sekine et al., 2012, Mol. Cell 48(5): 692--704 The Kelch Repeat Protein KLHDC10 Regulates Oxidative Stress-Induced ASK1 Activation by Suppressing PP5. [FBrf0220253] Sekine et al., 2011, J. Cell Sci. 124(17): 3006--3016 p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. [FBrf0215011]