FlyBase Allele Report: Dmel\Uvrag[B21]

General Information

Symbol

Dmel\Uvrag^B21

Species

D. melanogaster

Name

FlyBase ID

FBal0264046

Feature type

allele

Associated gene

Dmel\Uvrag

Associated Insertion(s)

Carried in Construct

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lee et al., 2011)

Mutagen

P-element activity

(Lee et al., 2011)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{SUPor-P}Uvrag^KG04163

(Lee et al., 2011)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of P{SUPor-P}Uvrag^KG04163 that deletes the first exon and most of the second exon of Uvrag.

(Lee et al., 2011)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Nagy et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Uvrag^B21 homozygous clones within a mosaic adult midgut epithelium show a severe increase in cell number compared to control clones.

(Nagy et al., 2016)

Homozygous mushroom body gamma neurons show a pruning defect, with the unpruned larval axons being retained into adulthood.

(Issman-Zecharya and Schuldiner, 2014)

Autolysosome formation appears normal in Uvrag^B21 mutant fat body clones in starved larvae. No accumulation of autophagosomes is seen. Autolysosomes are formed and are similar in shape to controls. However unlike controls more advanced stages of degradation are never seen in Uvrag^B21 mutant cells.

Eyes entirely composed of Uvrag^B21 mutant cells (generated using the GMR-hid method) are lighter in color compared to controls.

(Takáts et al., 2014)

~75% of Uvrag^KG04163/Uvrag^B7 or Uvrag^KG04163/Uvrag^B21 males show incomplete genitalia rotation, though the direction of rotation is wild type.

(Lee et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, enhanceable by Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Suppressed by

Statement

Reference

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, suppressible | partially by Scer\GAL4^Abd-B-LDN/N^dsRNA.P.UAS

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, suppressible | partially by mam^N.UAS/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

NOT suppressed by

Statement

Reference

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, non-suppressible by Egfr^GD1654/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, non-suppressible by Pvr^{dsRNA.UAS.cRa}/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, non-suppressible by ptc^NIG.2411R/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 has male genitalia phenotype, non-suppressible by N^ECN.UAS/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Uvrag^B21 is rescued by Scer\GAL4^NP5130/Uvrag^UAS.Tag:FLAG

(Nagy et al., 2016)

Uvrag^B21 is rescued by Scer\GAL4^da.G32/Uvrag^UAS.Tag:FLAG

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 is rescued by Uvrag^UAS.Tag:FLAG/Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 is rescued by Scer\GAL4^hs.PU/Uvrag^UAS.Tag:FLAG

(Lee et al., 2011)

Not rescued by

Uvrag^KG04163/Uvrag^B21 is not rescued by Scer\GAL4^elav.PU/Uvrag^UAS.Tag:FLAG

(Lee et al., 2011)

Uvrag^KG04163/Uvrag^B21 is not rescued by Scer\GAL4^Lsp2.PH/Uvrag^UAS.Tag:FLAG

(Lee et al., 2011)

Comments

The increased cell number observed in Uvrag^B21 adult midgut clones is suppressed by the expression of Uvrag^UAS.Tag:FLAG under the control of Scer\GAL4^esg-NP5130.

(Nagy et al., 2016)

Ubiquitous expression of Uvrag^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^da.G32 rescues the lethality of Uvrag^B21 homozygotes.

Expression of Uvrag^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Abd-B-LDN, but not Scer\GAL4^elav.PU or Scer\GAL4^Lsp2.PH, rescues the genital rotation defect of Uvrag^KG04163/Uvrag^B21 males.

Heat-shock induced expression of Uvrag^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^hs.PU, induced by a heat shock on day 7 +/- 2 days (late larval to early pupal stages), is sufficient to rescue the genital rotation defect of Uvrag^KG04163/Uvrag^B21 males.

(Lee et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

UVRAG²¹

(Issman-Zecharya and Schuldiner, 2014)

UVRAG^B21

(Hegedűs et al., 2016, Issman-Zecharya and Schuldiner, 2014, Lee et al., 2011)

UVRAG[B21]

(Takáts et al., 2014)

Uvrag^B21

(Nagy et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close