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General Information
Symbol
Scer\GAL4Clk.2341
Species
S. cerevisiae
Name
FlyBase ID
FBal0264191
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Clk856-gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A Clk fragment from -856 to +1485 (amino acid residues 1-18) is placed upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Elimination of molecular clock function (using cycΔ.UAS.Tag:MYC) in Scer\GAL4Clk.2341 cells in adults significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when expression is restricted to a smaller subset of cells using Scer\GAL80Pdf.PS.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Clk.2341 cells significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls; this is also the case when inhibition is restricted to a smaller subset of cells using Scer\GAL80Pdf.PS and when inhibition of this subset is restricted to the adult stage using a Gal80[ts] transgene.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and 627 nm (red) light) of cells expressing Scer\GAL4Clk.2341 with Ecol\lexAGMR19C05 or Ecol\lexAGMR43D05 in adults significantly reduces sleep, compared to controls; activation of cells expressing Scer\GAL4Clk.2341 with Ecol\lexAGMR73B06, Ecol\lexAGMR64A11, Ecol\lexAGMR81E08, Ecol\lexAGMR55C09, Ecol\lexAGMR19A11, Ecol\lexAGMR70E02 or Ecol\lexAGMR70G01 in adults does not significantly affect sleep, compared to controls; activation of cells expressing Scer\GAL4Clk.2341 with Ecol\lexAGMR66B05, Ecol\lexAGMR77H08, Ecol\lexAGMR12G04, Ecol\lexAGMR67F03, Ecol\lexAGMR65D05 or Ecol\lexAGMR18H11 in adults significantly increases sleep, compared to controls; activation of a subset of Scer\GAL4Clk.2341 and Ecol\lexAGMR16C05-expressing cells (restricted using Scer\GAL80cry.PS) in adults does not significantly affect sleep, compared to controls; activation of a subset of Scer\GAL4Clk.2341 and Ecol\lexAGMR18H11-expressing cells (restricted using Scer\GAL80cry.PS) in adults significantly reduces sleep, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4Clk.2341
Name Synonyms
Secondary FlyBase IDs
    References (15)