Allele Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}

General Information
Symbol	Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}	Species	H. sapiens
Name		FlyBase ID	FBal0264198
Feature type	allele	Associated gene	Hsap\APP

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Hua et al., 2011) in vitro construct - regulatory fusion (Hua et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an Aβ1-42 peptide of Hsap\APP fused to an N-terminal hh signal sequence. (Hua et al., 2011)
Carried in construct	P{UAS-Aβ42.hh} (Hua et al., 2011)
Tagged with	T:SS-hh (Hua et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective, with Scer\GAL4^elav.PLu (Hua et al., 2011) partially lethal - majority die, with Scer\GAL4^Act.PU (Hua et al., 2011) visible, with Scer\GAL4^GMR.PF (Hua et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Hua et al., 2011)
Detailed Description
	Statement Reference Expression of Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[GMR.PF] causes progressive eye degeneration correlated with age and expression levels. The eye distortion phenotype is enhanced by zinc or copper ion food supplements. The eye degeneration is ameliorated by zinc/copper and copper chelators. In control flies not expressing Hsap\APP[Aβ42.Scer\UAS.T:hh], normal eye structure is not affected by copper or zinc ion treatment. The eye degeneration phenotype caused by expression of Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[GMR.PF] is strongly enhanced by hydrogen peroxide at a dosage that does not affect wild-type flies. Expression of Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[Act.PU] reduces viability, evident as a lower eclosion rate. Viability is further reduced by dietary zinc ion supplement, but not by copper ion supplement or copper depletion. The decline in survival is counteracted by supplementing DP-109 (a Zn/Cu metal chelator) in the food, but not by supplementing another Cu-only specific chelator, BCS. When the expression of Hsap\APP[Aβ42.Scer\UAS.T:hh] is directed to the nervous system by using a neuron specific driver (Scer\GAL4[elav.PLu]), the flies exhibit defects in locomotion which is more severe upon dietary zinc ion supplementation, as measured by a standard climbing assay. Zinc ion chelators ameliorate this phenotype. (Hua et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by MTF-1^unspecified (Hua et al., 2011)
Suppressed by
Statement Reference Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by MTF-1^Scer\UAS.cHa, Scer\GAL4^GMR.PF (Hua et al., 2011) Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by MtnA^Scer\UAS.cHa, Scer\GAL4^GMR.PF (Hua et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of MTF-1[Scer\UAS.cHa] with Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[GMR.PF] ameliorates the eye phenotype caused by Hsap\APP[Aβ42.Scer\UAS.T:hh] expression alone. Co-expression of MtnA[Scer\UAS.cHa] with Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[GMR.PF] ameliorates the eye phenotype caused by Hsap\APP[Aβ42.Scer\UAS.T:hh] expression alone. MTF-1[unspecified] enhances the eye phenotype caused by expression of Hsap\APP[Aβ42.Scer\UAS.T:hh] under the control of Scer\GAL4[GMR.PF]. (Hua et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Hsap\APP^{Aβ42.Scer\UAS.T:hh} Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Hua et al., 2011, Biol. Chem. 392(10): 919--926 Toxicity of Alzheimer's disease-associated Aβ peptide is ameliorated in a Drosophila model by tight control of zinc and copper availability. [FBrf0215055]

Allele Hsap\APPAβ42.Scer\UAS.T:SS-hh

Allele Hsap\APP^{Aβ42.Scer\UAS.T:SS-hh}