Allele Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}

General Information
Symbol	Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}	Species	H. sapiens
Name		FlyBase ID	FBal0264223
Feature type	allele	Associated gene	Hsap\SPAST

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Du et al., 2010) in vitro construct - regulatory fusion (Du et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the full length (1.87kb) Hsap\SPAST cDNA, tagged at the 5' end with T:Avic\GFP[YFP.Venus]. (Du et al., 2010)
Carried in construct	P{UAS-SPAST.YFP} (Du et al., 2010)
Tagged with	T:Avic\GFP^YFP.Venus (Du et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of locomotor behavior defective \| adult stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of locomotor behavior defective \| adult stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010)
Other
Statement Reference Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype (Du et al., 2010) Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype (Du et al., 2010) Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype (Du et al., 2010)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of microtubule \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of NMJ bouton \| supernumerary \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of NMJ bouton \| supernumerary \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of microtubule \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of NMJ bouton \| supernumerary \| larval stage phenotype of spas^5.75 (Du et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a non-suppressor of microtubule \| larval stage phenotype of spas^5.75 (Du et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] under the control of Scer\GAL4[elav.Switch.PO] partially rescues the eclosion defects seen in spas[5.75] homozygotes. A similar level of rescue is seen when two copies of the transgene are expressed, however increasing the concentration of RU486 leads to increased lethality. The increased bouton number phenotype is partially rescued, with the number of boutons restored to close to wild type levels. The boutons seen are arranged linearly rather than being small and clustered as in spas[5.75]. Terminal bouton microtubule distribution is also rescued to close to wild type levels. Flies carrying either one or two copies of the transgene have lifespans of approximately 15 days. Co-expression of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] and Hsap\SPAST[R388.Scer\UAS.T:Avic\GFP-YFP.Venus] under the control of Scer\GAL4[elav.Switch.PO] partially rescues the eclosion defects seen in spas[5.75] homozygotes, although only 75% of flies eclose compared to when two copies of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] are expressed. The movement defects seen in spas[5.75] homozygotes are also partially rescued, with rescue flies still exhibiting mild walking rate, jumping and flying defects. The bouton number phenotype is partially rescued although less efficiently than when two copies of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] are expressed. Bouton number is reduced significantly compared to spas[5.75], but boutons seen are still small and clustered rather than linear. Terminal bouton microtubule distribution is not rescued. Flies of this genotype have a severely reduced lifespan of approximately 10 days. Co-expression of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] and Hsap\SPAST[L44.Scer\UAS.T:Avic\GFP-CFP] under the control of Scer\GAL4[elav.Switch.PO] restores the eclosion and movement defects seen in spas[5.75] homozygotes to a similar level as when two copies of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] are expressed. The spas[5.75] increased bouton number phenotype is partially rescued, although bouton number is slightly higher than when Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] is expressed alone. Terminal bouton microtubule distribution is also partially rescued. Flies of this genotype have an average lifespan of approximately 16 days. (Du et al., 2010)
Complementation & Rescue Data
Comments
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Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Du et al., 2010, Hum. Mol. Genet. 19(10): 1883--1896 Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia. [FBrf0210700]

Allele Hsap\SPASTScer\UAS.T:Avic\GFP-YFP.Venus

Allele Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}