Allele Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}

General Information
Symbol	Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}	Species	H. sapiens
Name		FlyBase ID	FBal0264225
Feature type	allele	Associated gene	Hsap\SPAST

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Du et al., 2010) in vitro construct - coding region fusion (Du et al., 2010) in vitro construct - regulatory fusion (Du et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a mutant form of Hsap\SPAST containing a nucleotide substitution (C256T) which results in an amino acid replacement (S44L). The construct is tagged with T:Avic\GFP[YFP.Venus] at the 5' end. (Du et al., 2010)
Carried in construct	P{UAS-SPAST.L44.CFP} (Du et al., 2010)
Tagged with	T:Avic\GFP^YFP.Venus (Du et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of locomotor behavior defective \| adult stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}/Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP} is a suppressor \| partially of eclosion defective phenotype of spas^5.75 (Du et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} is a non-suppressor of locomotor behavior defective \| adult stage phenotype of spas^5.75 (Du et al., 2010)
Other
Statement Reference Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype (Du et al., 2010) Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus}, Scer\GAL4^{elav.Switch.PO}, spas^5.75 has short lived phenotype (Du et al., 2010)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of microtubule \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{Scer\UAS.T:Avic\GFP-YFP.Venus} is a suppressor \| partially of NMJ bouton \| supernumerary \| larval stage phenotype of spas^5.75 (Du et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} is a non-suppressor of microtubule \| larval stage phenotype of spas^5.75 (Du et al., 2010) Scer\GAL4^{elav.Switch.PO}, Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}, Hsap\SPAST^{R388.Scer\UAS.T:Avic\GFP-YFP.Venus} is a non-suppressor of NMJ bouton \| supernumerary \| larval stage phenotype of spas^5.75 (Du et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] and Hsap\SPAST[L44.Scer\UAS.T:Avic\GFP-CFP] under the control of Scer\GAL4[elav.Switch.PO] restores the eclosion and movement defects seen in spas[5.75] homozygotes to a similar level as when two copies of Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] are expressed. The spas[5.75] increased bouton number phenotype is partially rescued, although bouton number is slightly higher than when Hsap\SPAST[Scer\UAS.T:Avic\GFP-YFP.Venus] is expressed alone. Terminal bouton microtubule distribution is also partially rescued. Flies of this genotype have an average lifespan of approximately 16 days. Expression of Hsap\SPAST[L44.Scer\UAS.T:Avic\GFP-CFP] under the control of Scer\GAL4[elav.Switch.PO] partially rescues the eclosion defects seen in spas[5.75] homozygotes. Co-expression of Hsap\SPAST[R388.Scer\UAS.T:Avic\GFP-YFP.Venus] and Hsap\SPAST[L44.Scer\UAS.T:Avic\GFP-CFP] under the control of Scer\GAL4[elav.Switch.PO] partially rescues the eclosion defects seen in spas[5.75], but fails to rescue the movement defects. Neither the increase in bouton number nor the terminal bouton microtubule distribution phenotype seen in spas[5.75] is rescued. Flies of this genotype have a severely reduced lifespan of approximately 4 days. (Du et al., 2010)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Du et al., 2010, Hum. Mol. Genet. 19(10): 1883--1896 Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia. [FBrf0210700]

Allele Hsap\SPASTL44.Scer\UAS.T:Avic\GFP-CFP

Allele Hsap\SPAST^{L44.Scer\UAS.T:Avic\GFP-CFP}