Open Close
General Information
Symbol
Dmel\stj1
Species
D. melanogaster
Name
FlyBase ID
FBal0264276
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

A13807993C

Amino acid change:

E133A | stj-PB; E133A | stj-PC; E133A | stj-PD

Reported amino acid change:

E133A

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: E133A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

stj1 mutant photoreceptor terminals show increased accumulation of autophagic vacuoles compared to controls.

Flies with homozygous visual systems (made using the eyFLP method) show a reduced depolarisation in response to light stimulus and a lack of "on-off" transients in electroretinogram recordings. Six photoreceptor terminals are seen per lamina cartridge in the flies, as occurs in wild type.

Flies in which homozygous clones have been induced in the presynaptic photoreceptors only (made using the ey3.5FLP system) show a lack on "on" transients in electroretinogram recordings.

stj1/Df(2R)Exel7128 animals generally arrest as pupae, with some animals emerging as uncoordinated adults.

stj1/Df(2R)Exel7128 mutants show seizure-like activity when recordings are taken from muscles 6/7 of dissected third instar larvae with intact ventral nerve cords at elevated temperature (36[o]C); although burst events are relatively rare, activity trains often last 30 seconds or longer. The mutant bursts are lower in amplitude than those of controls.

Evoked excitatory junctional potentials (EJPs) at the third instar larval neuromuscular junction have a reduced amplitude compared to controls in stj1/stj2, stj1/stj3 and stj1/Df(2R)Exel7128 animals. Ca[2+] cooperativity does not appear to be affected at stj1/Df(2R)Exel7128 synapses, but there is a reduction in synaptic Ca[2+] sensitivity, and the mutant synapses show increased facilitation compared with controls.

The amplitude of EJPs at the neuromuscular junction is normal in stj1/+ third instar larvae.

The mean amplitude, distribution of amplitude and frequency of spontaneous miniature EJPs at the larval neuromuscular junction are normal in stj1/Df(2R)Exel7128 animals.

Neuromuscular junctions of stj1/Df(2R)Exel7128 third instar larvae show an increase in synapse length/muscle area and an increase in bouton number/muscle area compared to controls. Ultrastructural analysis of individual boutons shows that there is no difference in vesicle density, number of active-zone associated vesicles or vesicle size in the mutants compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

stj1/Df(2R)Exel7128 flies expressing cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 have "on-off" transients in electroretinogram recordings.

The reduced excitatory junctional potential amplitude seen at the third instar larval neuromuscular junction in stj1/Df(2R)Exel7128 animals is partially suppressed by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155.

The hyperexcitability seen in recordings from muscles 6/7 of dissected third instar stj1/Df(2R)Exel7128 larvae with intact ventral nerve cords at elevated temperature (36[o]C) is reduced by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155; these animals rarely show activity bursts lasting 30 seconds or more.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

The chromosome may contain second site lethal mutation(s), as the lethal phase of homozygotes is earlier than that of hemizygotes or of transheterozygotes.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
  • FBal0240725
  • FBal0190393
References (4)