FB2025_01 , released February 20, 2025
Allele: Dmel\stj2
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General Information
Symbol
Dmel\stj2
Species
D. melanogaster
Name
FlyBase ID
FBal0264277
Feature type
allele
Associated gene
Dmel\stj
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ly et al., 2008)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q488term.

(Ly et al., 2008)

Contains a nonsense mutation which is predicted to result in a truncation after the VWA domain.

(Ly et al., 2006)

Amino acid replacement: ??term.

(Ly et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:13,810,515..13,810,515 [+]
Nucleotide change:

C13810515T

Amino acid change:

Q488term | stj-PB; Q488term | stj-PC; Q488term | stj-PD

Reported amino acid change:

Q488term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ly et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      stj2 heterozygous adults exhibit complete suppression of neuropathic sensitization after injury when compared to controls.

      (Khuong et al., 2019)

      stj2 mutant adults have no alterations in aversive shock or temperature olfactory memory, compared to controls.

      (Galili et al., 2014)

      Homozygous and stj2/Df(2R)Exel7128 larvae show a severely impaired thermal nociception response compared to controls.

      (Neely et al., 2010)

      Flies with homozygous visual systems (made using the eyFLP method) show a reduced depolarisation in response to light stimulus and a lack of "on-off" transients in electroretinogram recordings. Six photoreceptor terminals are seen per lamina cartridge in the flies, as occurs in wild type.

      Flies in which homozygous clones have been induced in the presynaptic photoreceptors only (made using the ey3.5FLP system) show a lack on "on" transients in electroretinogram recordings.

      stj2/Df(2R)Exel7128 animals generally arrest as pupae, with some animals emerging as uncoordinated adults.

      stj2/Df(2R)Exel7128 mutants show seizure-like activity when recordings are taken from muscles 6/7 of dissected third instar larvae with intact ventral nerve cords at elevated temperature (36[o]C); although burst events are relatively rare, activity trains often last 30 seconds or longer. The mutant bursts are lower in amplitude than those of controls.

      Evoked excitatory junctional potentials (EJPs) at the third instar larval neuromuscular junction have a reduced amplitude compared to controls in stj1/stj2 and stj2/Df(2R)Exel7128 animals. The amplitude of EJPs at the neuromuscular junction is normal in stj2/+ third instar larvae.

      The mean amplitude, distribution of amplitude and frequency of spontaneous miniature EJPs at the larval neuromuscular junction are normal in stj2/Df(2R)Exel7128 animals.

      Neuromuscular junctions of stj2/Df(2R)Exel7128 third instar larvae show an increase in synapse length/muscle area and an increase in bouton number/muscle area compared to controls. Ultrastructural analysis of individual boutons shows that there is no difference in vesicle density, number of active-zone associated vesicles or vesicle size in the mutants compared to controls.

      (Ly et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      stj2/Df(2R)Exel7128 has lethal phenotype, non-suppressible by Scer\GAL4Mhc.PW/cacUAS.EGFP

      (Ly et al., 2008)
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      stj2/Df(2R)Exel7128 flies expressing cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 have "on-off" transients in electroretinogram recordings.

      The reduced excitatory junctional potential amplitude seen at the third instar larval neuromuscular junction in stj2/Df(2R)Exel7128 animals is partially suppressed by expression of cacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155.

      (Ly et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      The chromosome may contain second site lethal mutation(s), as the lethal phase of homozygotes is earlier than that of hemizygotes or of transheterozygotes.

      (Ly et al., 2008)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      stj2
      (Khuong et al., 2019, Galili et al., 2014, Neely et al., 2010, Ly et al., 2008)
      stjMI
      α2δ2
      (Cunningham et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0240726
      • FBal0191037
      References (7)