FlyBase Allele Report: Dmel\mop[sfv3]

General Information

Symbol

Dmel\mop^sfv3

Species

D. melanogaster

Name

FlyBase ID

FBal0264533

Feature type

allele

Associated gene

Dmel\mop

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Gilbert et al., 2011)

Mutagen

ethyl methanesulfonate

(Gilbert et al., 2011)

Progenitor genotype

Cytology

Description

Contains a nonsense mutation in the seventh exon of mop, resulting in truncation within the C-terminal region, just prior to the PTPase catalytic region.

(Gilbert et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Gilbert et al., 2011)

lethal | recessive

(Gilbert et al., 2011)

Phenotype Manifest In

eye | somatic clone

(Gilbert et al., 2011)

Detailed Description

Statement

Reference

Mosaic mop^sfv3 adult eyes (generated using the eyFLP method) are small and rough and contain little to no mutant tissue.

(Gilbert et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^UASp.EGFP

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^CT.UASp.EGFP

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^UAS.cBa

(Gilbert et al., 2011)

NOT suppressed by

Statement

Reference

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/wts^UAS.Tag:MYC

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/ex^{linker.UASp.EGFP}

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/app^RNAi.UAS.cMa

(Gilbert et al., 2011)

Enhancer of

Statement

Reference

mop^sfv3, Scer\GAL4^GMR.PFa is an enhancer of abnormal size | adult stage phenotype of Hsap\YAP1^UAS.cHa, Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Other

Statement

Reference

Df(3L)H99, mop^sfv3 has abnormal mitotic cell cycle | somatic clone phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has lethal | pharate adult stage | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | adult stage | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has increased occurrence of cell division | somatic clone phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has abnormal size | somatic clone | adult stage phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has increased cell number | somatic clone | pupal stage phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has increased cell number | somatic clone | larval stage phenotype

(Gilbert et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^UAS.cBa

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^UASp.EGFP

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, suppressible by Scer\GAL4^Act.PU/ex^CT.UASp.EGFP

(Gilbert et al., 2011)

NOT suppressed by

Statement

Reference

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/wts^UAS.Tag:MYC

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/ex^{linker.UASp.EGFP}

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype, non-suppressible by Scer\GAL4^Act.PU/app^RNAi.UAS.cMa

(Gilbert et al., 2011)

Enhancer of

Statement

Reference

mop^sfv3 is an enhancer of eye phenotype of Hsap\YAP1^UAS.cHa, Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Other

Statement

Reference

Df(3L)H99, mop^sfv3 has eye | somatic clone phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has haltere | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has interommatidial cell | increased number | somatic clone | pupal stage phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has wing disc | somatic clone | third instar larval stage phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has eye | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has head | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has adult cuticle | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Df(3L)H99, mop^sfv3 has thorax | somatic clone - Minute background phenotype

(Gilbert et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Mosaic mop^sfv3 Df(3L)H99 adult eyes (generated using the eyFLP method) exhibit a clonal growth advantage compared to Df(3L)H99 controls.

Flies with heads composed entirely of mop^sfv3 Df(3L)H99 clone cells (generated in a minute background) die at the pharate adult stage with extra folds of head cuticle relative to controls. The eyes are constricted at their margins, protrude from the head and contain enlarged facets.

Generation of mop^sfv3 Df(3L)H99 mutant clones throughout the body (generated in a minute background) results in outgrowths in the thorax and increased haltere size compared to Df(3L)H99 mutants alone.

Excess interommatidial cells are seen in mop^sfv3 Df(3L)H99 pupal clones in comparison with Df(3L)H99 controls. Pupal clones also exhibit patterning defects.

Ectopic S phase cells are seen in mop^sfv3 Df(3L)H99 mutant eye clones posterior to the second mitotic wave. These cells are post-mitotic in both wild type and Df(3L)H99 controls.

mop^sfv3 Df(3L)H99 clones in the third instar larval wing disc epithelium are consistently larger, contain more cells than twin spots in controls and have regular boundaries. DNA content and size are also similar to Df(3L)H99 controls.

Expression of wts^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act.PU does not suppress the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^Act.PU suppresses the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of ex^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act.PU suppresses the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of ex^{CT.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act.PU suppresses the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of ex^{linker.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act.PU fails to suppress the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of app^{dsRNA.Scer\UAS.cMa} under the control of Scer\GAL4^Act.PU fails to suppress the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

(Gilbert et al., 2011)

Xenogenetic Interactions

Statement

Reference

:One copy of mop^sfv3 enhances the eye overgrowth phenotype seen when Hsap\YAP1^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PFa.

(Gilbert et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

F2.6.3

(Gilbert et al., 2011)

mop^sfv3

(Gilbert et al., 2011)

sfv³

(Gilbert et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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