Allele Dmel\p38b^Δ25

General Information
Symbol	Dmel\p38b^Δ25	Species	D. melanogaster
Name		FlyBase ID	FBal0264803
Feature type	allele	Associated gene	Dmel\p38b

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Vrailas-Mortimer et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	p38b^KG01337 (Vrailas-Mortimer et al., 2011)
Nature of the lesion	Statement Reference Excision of p38b[KG01337] results in the removal of 299bp upstream of the transcription start. (Vrailas-Mortimer et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	viable (Vrailas-Mortimer et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Mpk2¹, p38b^Δ25 has gravitaxis defective phenotype, suppressible by p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has locomotor behavior defective phenotype, suppressible by p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has oxidative stress response defective phenotype, suppressible \| partially by p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype, suppressible \| partially by Sod2^Scer\UAS.cMa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype, suppressible by p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has short lived phenotype, suppressible \| partially by p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011)
NOT suppressed by
Statement Reference Mpk2¹, p38b^Δ25 has gravitaxis defective phenotype, non-suppressible by Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has locomotor behavior defective phenotype, non-suppressible by Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by Cat^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by p38b^ala.Scer\UAS/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype, non-suppressible by Sod^Scer\UAS.cAa/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa (Vrailas-Mortimer et al., 2011)
Other
Statement Reference Mpk2¹, p38b^Δ25/p38b[+] has size defective \| pupal stage \| conditional phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25/p38b[+] has stress response defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has flight defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has gravitaxis defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has heat stress response defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has lethal \| pupal stage \| conditional phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has locomotor behavior defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has oxidative stress response defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has partially lethal - majority die phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has short lived phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has starvation stress response defective phenotype (Vrailas-Mortimer et al., 2011) Mpk2¹, p38b^Δ25 has stress response defective phenotype (Vrailas-Mortimer et al., 2011)
Phenotype Manifest In
Other
Statement Reference Mpk2¹, p38b^Δ25/p38b[+] has pupa \| conditional phenotype (Vrailas-Mortimer et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference p38b[Δ25] Mpk2[1] double mutant adults appear normal on eclosion but have a severely reduced lifespan compared to controls. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] significantly rescues the reduction in lifespan seen in p38b[Δ25] Mpk2[1] double mutants. No rescue is seen when p38b[Scer\UAS.cAa] is expressed in neurons under the control of Scer\GAL4[elav-C155]. p38b[Δ25] Mpk2[1] double mutant females exhibit age-dependent impairment in flight behaviour. Negative geotaxis is also prominently impaired and deteriorates more rapidly with age. Aberrant walking behaviour is seen, with 3 day old p38b[Δ25] Mpk2[1] double mutants exhibiting various distortions that include a tendency to drag the abdomen, dragging a leg, shuffling of the metathoracic legs and frequent slippage. Walking speed is reduced compared to controls and the improvement in walking ability with age seen in wild type flies does not occur. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] suppresses the geotaxis and walking defects seen in p38b[Δ25] Mpk2[1] double mutant females. No suppression is seen when p38b[Scer\UAS.cAa] is expressed pan-neuronally under the control of Scer\GAL4[elav-C155]. No age-dependent deterioration in the function of the giant-fiber system is observed in p38b[Δ25] Mpk2[1] double mutants. Almost all p38b[Δ25] Mpk2[1] 1-2 day old double mutant flies die within 24 hours of heat shock at 37[o]C for 5 hours, in contrast to an almost 100% survival rate in controls. When reared under conditions of dry starvation, 50% of p38b[Δ25] Mpk2[1] double mutant animals die within 15 hours, compared to 20% of controls. p38b[Δ25] Mpk2[1] double mutant flies are significantly more sensitive to hydrogen peroxide-induced oxidative stress compared to controls. Lifespan is also reduced when adult flies are exposed to the oxidising herbicide paraquat. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] significantly rescues the increased sensitivity to hydrogen peroxide-induced oxidative stress seen in p38b[Δ25] Mpk2[1] double mutants. Only 17% of p38b[Δ25] Mpk2[1] double mutant flies make it to adulthood compared to controls. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] completely rescues the reduced viability seen in p38b[Δ25] Mpk2[1] double mutants. Expression of p38b[ala.Scer\UAS] in muscles under the control of Scer\GAL4[Mef2.PR] does not suppress the reduction in viability seen in p38b[Δ25] Mpk2[1] double mutants. Expression of Sod2[Scer\UAS.cMa] under the control of Scer\GAL4[Mef2.PR] partially rescues the reduced viability seen in p38b[Δ25] Mpk2[1] double mutants. Expression of Sod[Scer\UAS.cAa] under the control of Scer\GAL4[Mef2.PR] does not rescue the reduced viability seen in p38b[Δ25] Mpk2[1] double mutants. Expression of Cat[Scer\UAS.cAa] under the control of Scer\GAL4[Mef2.PR] does not rescue the reduced viability seen in p38b[Δ25] Mpk2[1] double mutants. p38b[Δ25] Mpk2[1] double mutants fail to eclose when grown under chronic hypoxic conditions. Lifespan is equally short in hypoxic and normoxic conditions. Homozygous Mpk2[1] mutants with one copy of p38b[Δ25] have smaller pupae and adults under hypoxic conditions compared to normoxia. (Vrailas-Mortimer et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	p38b^Δ25 p38Kb^Δ25 (Vrailas-Mortimer et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 A Muscle-Specific p38 MAPK/Mef2/MnSOD Pathway Regulates Stress, Motor Function, and Life Span in Drosophila. [FBrf0216446]

Allele Dmel\p38bΔ25

Allele Dmel\p38b^Δ25