The strongly reduced eye size characteristic for adult flies expressing hidGMR.PG is partially reverted by expression of either DubaGD11813 or DubaKK108478 under the control of Scer\GAL4GMR.PU or by combination with the Duba107 allele in homozygous state.
Drice17/Drice17, Drice17/DriceΔ1, Drice17/DriceL1, Drice17/DriceL2, Drice17/DriceC1, Drice17/DriceC2, Drice17/DriceS1, Drice17/DriceS2 (but not Drice17/+) significantly suppresses the eye ablation phenotype in hidGMR.PG flies. DriceL1/+ weakly suppresses the eye ablation phenotype in hidGMR.PG flies.
Expression of RetGMR.PR partially suppresses the reduction in eye size seen in flies expressing hidGMR.PG. This suppression is partially reversed when FakScer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.
Eyes mosaic for cosH29, cosL51, cosP50, ptcC, ptcL49, Pka-C1K2 or Pka-C1B3 (generated using the ey-FLP/FRT system) moderately suppress the WGMR.PG eye phenotype. This suppression is cell non-autonomous.
Eye discs mosaic for cosH29 (generated using the ey-FLP/FRT system) suppress the WGMR.PG cell death phenotype by increasing the apoptosis resistance of neighbouring non-mutant cells - cosH29 clones themselves are unprotected from WGMR.PG-induced apoptosis. This cell non-autonomous suppression occurs only in the first, and not the second, apoptotic wave.
The suppression of the WGMR.PG eye phenotype seen in eyes mosaic for cosH29 (generated using the ey-FLP/FRT system) is reverted by expression of ciCe-2.Scer\UAS.T:Ivir\HA1 (via the MARCM system) in the cosH29 clones.
Early third instar larvae expressing shiK44A.Scer\UAS under the control of "BL-Gal4" (a combination of Scer\GAL4elav.PLu, Scer\GAL80tsh-GAL80 and Scer\GAL80Cha.PK) at the restrictive temperature and also carrying WGMR.PG show a normal avoidance response to a spot of intense light.
Co-expression of AckScer\UAS.cSb under the control of Scer\GAL4GMR.PU suppresses the increased number of apoptotic cells seen posterior to the second mitotic wave and in the posterior region of the eye disc in the zone of compensatory proliferation in eye discs expressing WGMR.PG.
Co-expression of nitodsRNA.Scer\UAS.cCa and spendsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF partially suppresses the reduction in eye size seen in flies expressing WGMR.PG. The suppression is greater than is seen when nitodsRNA.Scer\UAS.cCa is expressed alone.
The small eye phenotype caused by WGMR.PG is moderately strongly suppressed by inducing homozygous clones of Uba1D6 in the eye. A reduction in apoptotic cell death is seen in the double mutant eye discs compared to WGMR.PG single mutants, with the suppression occurring in a region specific manner.
The small eye phenotype caused by WGMR.PG is moderately strongly suppressed by inducing homozygous clones of Uba1H33 or Uba1H42 in the eye. The rescued eye tissue is homozygous for the Uba1 mutation in each case, indicating that the suppression is cell autonomous.
WGMR.PG-induced ablation of the visual system results in rhythmic locomotory activity behaviour in flies expressing Scer\GAL4P2.4.Pdf driven crydsRNA.Scer\UAS.cPa under constant light conditions. Wild-type flies do not show rhythmic behavior under the same conditions.
The WGMR.PG eye ablation phenotype is strongly suppressed when eyes carry homozygous clones for the following mutations: ArkA3b, ArkA5, ArkA24, ArkB5, ArkB15, ArkC1, ArkC4, ArkD3, ArkD4, ArkE4, ArkG8, ArkG15, ArkG29, ArkH16, ArkH28, ArkH36, ArkJ15, ArkL46, ArkN28, ArkN52, ArkP13, ArkP15, ArkP46, ArkP56, ArkS7, ArkS9.
A WGMR.PG background, used to eliminate all heterozygous and wild-type cells in the developing eye, Upf214J cells proliferate, differentiate, and form an eye, although the eyes are smaller and more disorganised (rougher) than those of controls.
The small, rough eye phenotype of WGMR.PG flies is partially suppressed by the expression of mir-278KN448.mirvana-1.Scer\UAS under the control of Scer\GAL4GMR.PF and strongly suppressed by mir-278KN447.Scer\UAS under the control of Scer\GAL4GMR.PF.
The suppression is even stronger in Ice17 homozygous flies.
Eye size is largely restored by co-expression of banEP3622 (driven by Scer\GAL4GMR.PF) with WGMR.PG. The addition of Df(3L)banΔ1 to WGMR.PG animals leads to a significant enhancement of the eye phenotype.
The addition of Icefl.GMR to WGMR.PG produces no enhancement of either phenotype instead producing an additive effect. The addition of WGMR.PG to Dcp-1fl.GMR flies show a weak enhancement of the eye phenotype seen in these flies.