FlyBase Allele Report: Dmel\Sdc[2639]

General Information

Symbol

Dmel\Sdc²⁶³⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0265128

Feature type

allele

Associated gene

Dmel\Sdc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-in gene targeting

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Schulz et al., 2011)

Progenitor genotype

Cytology

Description

Deletion of guanine at nucleotide position 89 of Sdc exon 3, resulting in a frameshift followed by a premature stop codon.

(Schulz et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:21,412,330..21,412,330 [-]

Comment:

Deletion of a G residue in exon 3 leads to a frameshift and early translation termination. Mutant created by end in homogous recombination.

(Schulz et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Stork et al., 2014)

partially lethal - majority die, with Scer\GAL4^btl.PS

(Schulz et al., 2011)

partially lethal - majority die (with Df(2R)Exel6067)

(Schulz et al., 2011)

partially lethal - majority die (with Df(2R)PK1)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²³)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc⁴⁸)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc⁹⁷)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁴⁵¹)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁵²³)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁵²⁷)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁶²²)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁶²⁶)

(Schulz et al., 2011)

partially lethal - majority die | recessive

(Schulz et al., 2011)

viable (with Df(2R)PI12)

(Schulz et al., 2011)

viable (with Df(2R)XE-2900)

(Schulz et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant embryos show mild defects in the infiltration of astrocyte processes into the neuropil and in migration of the ventral-most astrocyte.

(Stork et al., 2014)

Adult Sdc²⁶³⁹ escapers have no obvious external phenotypes. However in third instar larvae the 10 dorsal branches of the tracheal system often fail to establish a dorsal anastomosis at the midline, a phenotype that is rarely seen in wild type or heterozygotes. Ganglionic branch number and length are also reduced. The frequency of missing anastomoses varies between segments, but overall almost half of all dorsal branches fail to fuse. The fusion failures can be divided into three distinct phenotypic categories; failure of dorsal branches to meet due to anterior-posterior axis guidance defects, dorsal branches that reach the midline but fail to fuse, and dorsal branches that are incomplete or completely absent. Removal of the maternal gene product results in an enhanced phenotype.

Sdc²⁶³⁹ homozygous third instar larvae have a reduced number of dorsal branch cells compared to wild type and heterozygotes. Stalk cells, terminal cells and fusion cells are all affected. In dorsal branches in which terminal cells are present the terminal extensions are not significantly reduced. The loss of different cell types is not random but occurs in order (two stalk cells followed by the terminal cell, the fusion cell and then the final two stalk cells).

In Sdc²⁶³⁹/Sdc²³ third instar larvae the 10 dorsal branches of the tracheal system fail to establish a dorsal anastomosis at the midline.

In the majority of Sdc²⁶³⁹ single fusion cell clones (15/16) dorsal branch fusion occurs normally.

(Schulz et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Sara^Ubi.PJ, Sdc⁴⁸/Sdc²⁶³⁹ has partially lethal - majority die phenotype

(Schulz et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sdc²⁶³⁹ has adult dorsal tracheal branch | third instar larval stage phenotype, suppressible | partially by sli²/sli[+]

(Schulz et al., 2011)

NOT suppressed by

Statement

Reference

Sdc²⁶³⁹ has adult dorsal tracheal branch | third instar larval stage phenotype, non-suppressible by lea[+]/robo2^S4-14

(Schulz et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of sli² partially suppresses the dorsal branch fusion phenotype seen in the tracheal system of Sdc²⁶³⁹ homozygous third instar larvae. One copy each of sli² and Sdc²⁶³⁹ does not produce any dorsal branch fusion defects.

One copy of lea^S4-14 fails to suppress the dorsal branch fusion phenotype seen in the tracheal system of Sdc²⁶³⁹ homozygous third instar larvae. One copy each of lea^S4-14 and Sdc²⁶³⁹ does not produce any dorsal branch fusion defects.

(Schulz et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sdc²⁶³⁹ is rescued by Sdc^RESC.ΔC.UAS/Scer\GAL4^btl.PS

(Schulz et al., 2011)

Partially rescued by

Sdc²⁶³⁹ is partially rescued by Sdc^RESC.UAS/Scer\GAL4^btl.PS

(Schulz et al., 2011)

Comments

Expression of Sdc^{RESC.Scer\UAS} under the control of Scer\GAL4^btl.PS rescues the loss of dorsal branch fusion seen in Sdc²⁶³⁹ mutants, but fails to rescue the semi-lethality.

Expression of Sdc^{RESC.ΔC.Scer\UAS} under the control of Scer\GAL4^btl.PS rescues the loss of dorsal branch fusion seen in Sdc²⁶³⁹ mutants.

(Schulz et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sdc²⁶³⁹

dSdc FS

(Schulz et al., 2011)

sdc²⁶³⁹

(Stork et al., 2014, Schulz et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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