Allele Dmel\sfl^Scer\UAS.cKa

General Information
Symbol	Dmel\sfl^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kamimura	FlyBase ID	FBal0265577
Feature type	allele	Associated gene	Dmel\sfl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kamimura et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS drives expression of a sfl cDNA. (Kamimura et al., 2011)
Carried in construct	P{UAS-sfl.K} (Kamimura et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die, with Scer\GAL4^Act.PU (Kamimura et al., 2011) partially lethal - majority die, with Scer\GAL4^hh.PU (Kamimura et al., 2011) size defective, with Scer\GAL4^hh.PU (Kamimura et al., 2011)
Phenotype Manifest In
	anterior crossvein, with Scer\GAL4^hh.PU (Kamimura et al., 2011) wing disc, with Scer\GAL4^hh.PU (Kamimura et al., 2011) wing margin, with Scer\GAL4^hh.PU (Kamimura et al., 2011)
Detailed Description
	Statement Reference Expression of sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system. Expression of sfl[Scer\UAS.cKa] using Scer\GAL4[hs.PU] and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality. Expression of sfl[Scer\UAS.cKa] with Scer\GAL4[Act.PU] results in 83% lethality and with Scer\GAL4[hh.PU] results in 96% lethality. Expression of sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in posterior wing margin notching in 13% of flies and loss of the anterior crossvein in 25% of flies. Expression of sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] significantly decreases the size of the posterior compartment of the wing disc. (Kamimura et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^Act.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype, suppressible by Hs2st^Scer\UAS.cKa, Scer\GAL4^Act.PU (Kamimura et al., 2011) Scer\GAL4^Act.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype, suppressible by Hsepi^Scer\UAS.cKa, Scer\GAL4^Act.PU (Kamimura et al., 2011) Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype, suppressible by Hs2st^Scer\UAS.cKa, Scer\GAL4^hh.PU (Kamimura et al., 2011) Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype, suppressible by Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU (Kamimura et al., 2011)
Other
Statement Reference Hs6st^Scer\UAS.cKa, Scer\GAL4^Act.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has lethal phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has size defective phenotype (Kamimura et al., 2011)
Phenotype Manifest In
Other
Statement Reference Hs2st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has wing margin phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has wing disc phenotype (Kamimura et al., 2011) Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has anterior crossvein phenotype (Kamimura et al., 2011) Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has wing margin phenotype (Kamimura et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Simultaneous expression of Hs6st[Scer\UAS.cKa] and sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system. Simultaneous expression of Hs6st[Scer\UAS.cKa] and sfl[Scer\UAS.cKa] using Scer\GAL4[hs.PU] and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality. Co-expression of Hsepi[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] results in 58% lethality. Co-expression of Hs2st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] results in 24% lethality. Co-expression of Hs6st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] results in 69% lethality. Co-expression of Hsepi[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in 39% lethality. Co-expression of Hs2st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in 0% lethality. Co-expression of Hs6st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in 100% lethality. Co-expression of Hsepi[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in posterior wing margin notching in 5% of flies and loss of the anterior crossvein in 2% of flies. Co-expression of Hs2st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in posterior wing margin notching in 3% of flies. Co-expression of sfl[Scer\UAS.cKa] and Hs6st[Scer\UAS.cKa] using Scer\GAL4[hh.PU] significantly decreases the size of the posterior compartment of the wing disc. (Kamimura et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	sfl^Scer\UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kamimura
Secondary FlyBase IDs

References ( 1 )
Research paper	Kamimura et al., 2011, Glycobiology 21(5): 607--618 In vivo manipulation of heparan sulfate structure and its effect on Drosophila development. [FBrf0213406]

Allele Dmel\sflScer\UAS.cKa

Allele Dmel\sfl^Scer\UAS.cKa