Allele Dmel\Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}

General Information
Symbol	Dmel\Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0265626
Feature type	allele	Associated gene	Dmel\Ran

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Cesario and McKim, 2011) in vitro construct - coding region fusion (Cesario and McKim, 2011) in vitro construct - regulatory fusion (Cesario and McKim, 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of a GDP-locked form of ran (amino acid replacement T24N) which is tagged at the N-terminal end with three copies of T:Ivir\HA1. (Cesario and McKim, 2011)
Carried in construct	P{UASp-ran.T24N} (Cesario and McKim, 2011)
Tagged with	T:Ivir\HA1 (Cesario and McKim, 2011)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Cesario and McKim, 2011) lethal, with Scer\GAL4^αTub84B.PL (Cesario and McKim, 2011) male sterile, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Cesario and McKim, 2011)
Phenotype Manifest In
	karyosome, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Cesario and McKim, 2011) meiotic spindle, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Cesario and McKim, 2011)
Detailed Description
	Statement Reference Expression of ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] results in a drastic reduction in female fertility, with an average of only 6.8 progeny per female (compared to 62.1 per wild-type female). Males expressing ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] are completely sterile. Defects in spindle and karyosome morphology are often seen in oocytes expressing ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16]. The microtubules are often not tapered at the spindle poles and the chromosomes are often disorganised and fail to condense into a single round or oval karyosome. Chiasmate chromosome segregation is not affected in females expressing ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16]. However, the segregation of achiasmate chromosomes is disrupted in these animals. The majority of zygotes derived from females expressing ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] arrest development without any evidence of embryonic mitotic divisions. Two types of zygote are seen. Approximately half contain the unfused female and male meiotic products and lack organised microtubules, while the remaining half have no visible nuclei. (Cesario and McKim, 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} has female sterile phenotype, suppressible \| partially by sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Cesario and McKim, 2011)
Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} is a suppressor \| partially of female sterile phenotype of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} (Cesario and McKim, 2011)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} is a suppressor of oocyte phenotype of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} (Cesario and McKim, 2011) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} is a suppressor of spindle \| ectopic phenotype of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} (Cesario and McKim, 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] and sub[ΔNT.Scer\UAS.P\T.T:Avic-EGFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] increases female fertility compared to the fertility of females expressing either ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] or sub[ΔNT.Scer\UAS.P\T.T:Avic-EGFP] separately. The ectopic spindle phenotype seen in the oocytes of females expressing sub[ΔNT.Scer\UAS.P\T.T:Avic-EGFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] is completely suppressed by co-expression of ran[T24N.Scer\UAS.P\T.T:Ivir\HA1]. The arrest in embryonic development seen in zygotes derived from females expressing ran[T24N.Scer\UAS.P\T.T:Ivir\HA1] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] is partially suppressed by co-expression of sub[ΔNT.Scer\UAS.P\T.T:Avic-EGFP]. (Cesario and McKim, 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} ran^T24N (Cesario and McKim, 2011)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Cesario and McKim, 2011, J. Cell Sci. 124(22): 3797--3810 RanGTP is required for meiotic spindle organization and the initiation of embryonic development in Drosophila. [FBrf0216751]

Allele Dmel\RanT24N.Scer\UAS.P\T.T:Ivir\HA1

Allele Dmel\Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}