Allele Dmel\Pdk1³³

General Information
Symbol	Dmel\Pdk1³³	Species	D. melanogaster
Name		FlyBase ID	FBal0265719
Feature type	allele	Associated gene	Dmel\Pdk1

Allele class
Mutagen	Delta2-3
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Cheng et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{SUPor-P}Pdk1^KG01447 (Cheng et al., 2011)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion. Approximately 600bp of the original insertion remain. (Cheng et al., 2011)
Caused by insertion	P{?SUPor-P}Pdk1³³ (Cheng et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| third instar larval stage (Cheng et al., 2011) partially lethal - majority die (Cheng et al., 2011) small body \| adult stage (Cheng et al., 2011)
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage (Cheng et al., 2011) motor neuron \| larval stage (Cheng et al., 2011) NMJ bouton \| third instar larval stage (Cheng et al., 2011)
Detailed Description
	Statement Reference Homozygotes survive to the third instar larval stage and to the adult stage in reduced numbers. Homozygous adults show a severe reduction in body size compared to controls. Motoneuron cell bodies are smaller in size than normal in homozygous larvae. Absolute bouton number at the neuromuscular junction is normal in homozygous third instar larvae, while there is a 35% decrease in bouton size. The muscle fibres at the neuromuscular junction are 35% smaller than normal. As a result, normalised bouton number/muscle area at the mutant neuromuscular junction is increased by 55% compared to wild type. The number of active zones and the total synaptic area is significantly reduced at the mutant neuromuscular junction. (Cheng et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Pdk1³³ has neuroanatomy defective \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^VGlut-OK371/S6k^{STDETE.Scer\UAS} (Cheng et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference Pdk1³³ has NMJ bouton \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^VGlut-OK371/S6k^{STDETE.Scer\UAS} (Cheng et al., 2011)
NOT suppressed by
Statement Reference Pdk1³³ has NMJ bouton \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/S6k^Scer\UAS.cCa (Cheng et al., 2011)
Other
Statement Reference Pdk1³³, S6k^{STDETE.Scer\UAS}, Scer\GAL4^VGlut-OK371 has NMJ bouton \| third instar larval stage phenotype (Cheng et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of S6k[STDETE.Scer\UAS] under the control of Scer\GAL4[VGlut-OK371] partially rescues the reduction in bouton size at the neuromuscular junction which is seen in Pdk1[33] larvae. In addition, the number of boutons at the neuromuscular junction is increased compared to wild type. Expression of S6k[Scer\UAS.cCa] under the control of Scer\GAL4[VGlut-OK371] does not rescue the reduction in bouton size at the neuromuscular junction which is seen in Pdk1[33] larvae. (Cheng et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Pdk1³³ is partially rescued by Pdk1^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^C57 (Cheng et al., 2011) Pdk1³³ is partially rescued by Scer\GAL4^VGlut-OK371/Pdk1^{Scer\UAS.T:Zzzz\FLAG} (Cheng et al., 2011)
Comments	Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[VGlut-OK371] fully restores the defects in bouton size which are seen at the Pdk1[33] neuromuscular junction, but the defects in muscle size that are also seen at the Pdk1[33] neuromuscular junction are not rescued. Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[C57] fully restores the defects in muscle size which are seen at the Pdk1[33] neuromuscular junction, but the defects in bouton size that are also seen at the Pdk1[33] neuromuscular junction are not rescued. (Cheng et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Pdk1³³ PDK1³³ (Cheng et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Cheng et al., 2011, J. Cell Biol. 194(6): 921--935 S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development. [FBrf0216911]

Allele Dmel\Pdk133

Allele Dmel\Pdk1³³