|Feature type||allele||Associated gene||Dmel\Pdk1|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Imprecise excision of the progenitor insertion. Approximately 600bp of the original insertion remain.
|Caused by insertion|
|Phenotype Manifest In|
Homozygotes survive to the third instar larval stage and to the adult stage in reduced numbers. Homozygous adults show a severe reduction in body size compared to controls. Motoneuron cell bodies are smaller in size than normal in homozygous larvae. Absolute bouton number at the neuromuscular junction is normal in homozygous third instar larvae, while there is a 35% decrease in bouton size. The muscle fibres at the neuromuscular junction are 35% smaller than normal. As a result, normalised bouton number/muscle area at the mutant neuromuscular junction is increased by 55% compared to wild type. The number of active zones and the total synaptic area is significantly reduced at the mutant neuromuscular junction.
|Phenotype Manifest In|
|NOT suppressed by|
Expression of S6k[STDETE.Scer\UAS] under the control of Scer\GAL4[VGlut-OK371] partially rescues the reduction in bouton size at the neuromuscular junction which is seen in Pdk1 larvae. In addition, the number of boutons at the neuromuscular junction is increased compared to wild type. Expression of S6k[Scer\UAS.cCa] under the control of Scer\GAL4[VGlut-OK371] does not rescue the reduction in bouton size at the neuromuscular junction which is seen in Pdk1 larvae.
|Complementation & Rescue Data|
|Partially rescued by|
Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[VGlut-OK371] fully restores the defects in bouton size which are seen at the Pdk1 neuromuscular junction, but the defects in muscle size that are also seen at the Pdk1 neuromuscular junction are not rescued. Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[C57] fully restores the defects in muscle size which are seen at the Pdk1 neuromuscular junction, but the defects in bouton size that are also seen at the Pdk1 neuromuscular junction are not rescued.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 1 )|