Allele Dmel\Pdk1^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Pdk1^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0265720
Feature type	allele	Associated gene	Dmel\Pdk1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Cheng et al., 2011) in vitro construct - regulatory fusion (Cheng et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the Pdk1 open reading frame which is tagged at the N-terminal end with three copies of T:Zzzz\FLAG. (Cheng et al., 2011)
Carried in construct	P{UAS-Pdk1.FLAG} (Cheng et al., 2011)
Tagged with	T:Zzzz\FLAG (Cheng et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| third instar larval stage, with Scer\GAL4^tub.PU (Cheng et al., 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^VGlut-OK371 (Cheng et al., 2011)
Phenotype Manifest In
	NMJ bouton \| supernumerary \| third instar larval stage, with Scer\GAL4^tub.PU (Cheng et al., 2011) NMJ bouton \| supernumerary \| third instar larval stage, with Scer\GAL4^VGlut-OK371 (Cheng et al., 2011)
Detailed Description
	Statement Reference Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of either Scer\GAL4[VGlut-OK371] or Scer\GAL4[tub.PU] increases the absolute bouton number at the third larval instar neuromuscular junction. Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[VGlut-OK371] results in an increase in the number of active zones, an increase in the total synaptic area and a small increase in active zone density at the third larval instar neuromuscular junction. (Cheng et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference Scer\GAL4^VGlut-OK371/Pdk1^{Scer\UAS.T:Zzzz\FLAG} is a non-suppressor of neuroanatomy defective \| third instar larval stage phenotype of Df(3L)CH18/S6k^l-1 (Cheng et al., 2011)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Scer\GAL4^VGlut-OK371/Pdk1^{Scer\UAS.T:Zzzz\FLAG} is a non-suppressor of NMJ bouton \| third instar larval stage phenotype of Df(3L)CH18/S6k^l-1 (Cheng et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[VGlut-OK371] does not rescue the reduction in bouton size which is seen at the neuromuscular junction in S6k[l-1]/Df(3L)CH18 third instar larvae. (Cheng et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Pdk1^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^C57 partially rescues Pdk1³³ (Cheng et al., 2011) Scer\GAL4^VGlut-OK371/Pdk1^{Scer\UAS.T:Zzzz\FLAG} partially rescues Pdk1³³ (Cheng et al., 2011)
Comments	Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[VGlut-OK371] fully restores the defects in bouton size which are seen at the Pdk1[33] neuromuscular junction, but the defects in muscle size that are also seen at the Pdk1[33] neuromuscular junction are not rescued. Expression of Pdk1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[C57] fully restores the defects in muscle size which are seen at the Pdk1[33] neuromuscular junction, but the defects in bouton size that are also seen at the Pdk1[33] neuromuscular junction are not rescued. (Cheng et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Pdk1^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Cheng et al., 2011, J. Cell Biol. 194(6): 921--935 S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development. [FBrf0216911]

Allele Dmel\Pdk1Scer\UAS.T:Zzzz\FLAG

Allele Dmel\Pdk1^{Scer\UAS.T:Zzzz\FLAG}