FB2025_01 , released February 20, 2025
Allele: Dmel\caz383
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General Information
Symbol
Dmel\caz383
Species
D. melanogaster
Name
FlyBase ID
FBal0265755
Feature type
allele
Associated gene
Dmel\caz
Associated Insertion(s)
Carried in Construct
Also Known As
caz1
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Wang et al., 2011)
Progenitor genotype
P{EP}cazEP1564
(Wang et al., 2011)
Caused by aberration
Df(1)383
Cytology
Description

Imprecise excision of P{EP}cazEP1564 resulting in a deletion that removes 2443 bp of the caz coding sequence and 3251 bp of the upstream region including the entire caz promoter. The deletion also disrupts CG32576.

(Wang et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:16,285,595..16,291,288 [+]
Comment:

Approximate boundaries of the caz383 deletion, which was reported as a small deletion that removes 2,443 bp of the caz gene and 3,251 bp of upstream sequences including the entire caz promoter. The boundaries are approximate because it's not clear what coordinate was used as the beginning of the caz gene. The deletion was mapped from a transcription start at X:16182879 (R5 coordinates).

(Wang et al., 2011)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis
      CEA with CG32576+t5.0
      (Wang et al., 2011)
      CEA
      (Baldwin et al., 2016)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      caz383 homozygotes are semi-lethal and the few eclosing adults are not able to climb. These mutants also show impaired larval locomotion in crawling assays, as compared to controls.

      caz383 homozygotes show a decrease in the axonal transport both of mitochondria and of (NPY-positive) vesicles (i.e. significant increases in stationary fraction) in third instar larval motor neurons, as compared to controls.

      (Baldwin et al., 2016)

      Third instar larvae which are mutant for 'caz1' (FlyBase curator comment: this mutant corresponds to Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) display increased evoked excitatory junction potential (EJP) amplitude compared with controls. This increase is due to an approximately 25% increase in mEJP amplitude without affecting quantal content. The mutants have normal mEJP frequency. Active zones of caz1 mutant animals appear normal.

      (Machamer et al., 2014)

      The neuromuscular junctions of 'caz1' mutants (FlyBase curator comment: this mutant corresponds to Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) experience a decrease in the amplitude of excitatory junctional currents (EJCs), compared to wild-type. The rate of EJC decay in markedly increased in caz1 mutants compared to controls (decay time constants are approximately 35% of those in controls). This substantial change in decay kinetics, in combination with the decrease in EJC amplitude, results in an even more profound decrease in the integrated synaptic current.

      (Shahidullah et al., 2013)

      Male larvae hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) are morphologically normal. However, only 14% of these animals eclose as adults.

      caz1/Y adult escapers show a 57% decrease in average life span compared to controls. They also show a number of external defects, including a mild rough eye phenotype, abnormal genitalia and defects in both bristles and wing vein organisation.

      caz1/Y adult escapers have prominent locomotion defects. Most are unable to fly, and compared to controls, they walk slowly, often fall over, and have difficulty righting themselves. They show a 55.9% decrease in climbing ability in a negative geotaxis assay abd a 67.6% decrease in locomotor speed compared to controls.

      The brains of 25 day old caz1/Y adult escapers do not show evidence of vacuolization or other evidence of extensive neuronal death.

      (Wang et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      caz383 has partially lethal - majority die phenotype, suppressible by Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Baldwin et al., 2016)

      caz383 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Baldwin et al., 2016)

      caz383 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible | partially by Hsap\FUSUAS.10x.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Baldwin et al., 2016)

      caz383 has partially lethal - majority die phenotype, suppressible | partially by Hsap\FUSP525L.10x.UAS.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Baldwin et al., 2016)

      CG32576+t5.0, caz383 has short lived phenotype, suppressible | partially by Scer\GAL4elav-C155/Hsap\FUSP525L.10x.UAS.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has short lived phenotype, suppressible | partially by Scer\GAL4elav-C155/Hsap\FUSR522G.10x.UAS.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has partially lethal - majority die phenotype, suppressible by Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has partially lethal - majority die phenotype, suppressible by Scer\GAL4elav-C155/Hsap\FUSP525L.10x.UAS.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has partially lethal - majority die phenotype, suppressible by Scer\GAL4elav-C155/Hsap\FUSR522G.10x.UAS.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has abnormal locomotor behavior phenotype, suppressible | partially by Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has abnormal locomotor behavior phenotype, suppressible | partially by Scer\GAL4elav-C155/Hsap\FUSP525L.10x.UAS.Tag:FLAG

      (Wang et al., 2011)

      CG32576+t5.0, caz383 has short lived phenotype, suppressible by Scer\GAL4elav-C155/Hsap\FUSUAS.10x.Tag:FLAG

      (Wang et al., 2011)
      NOT suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of TBPHScer\UAS.10x.T:Avic\GFP-YFP.Venus in the nervous system under the control of Scer\GAL4elav-C155 does not rescue the reduced viability, locomotion defects or reduced longevity of adult males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function).

      The neuromuscular junction expansion induced by the overexpression of TBPHScer\UAS.10x.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4RapGAP1-OK6 is completely suppressed in a 'caz1'/Y background.

      (Wang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference

      The decreased axonal transport of mitochondria in caz383 homozygous third instar larval motor neurons is rescued by the expression of either Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG or Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI; their decreased axonal transport of (NPY-positive) vesicles is rescued by the expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG, but not or Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4CCAP.PI.

      The semi-lethality, the decreased third instar larval crawling capacity and the decreased adult climbing capacity observed in caz383 homozygotes are fully/nearly fully suppressed by the expression of either Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG or Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42.

      (Baldwin et al., 2016)

      Expression of either Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG, Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG or Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG in the nervous system under the control of Scer\GAL4elav-C155 almost completely rescues the adult viability of males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) - the level of rescue seen with each Hsap\FUS transgene is not significantly different from that seen when a transgene containing wild-type caz is expressed using the same driver.

      Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 strongly rescues the locomotor defects seen in caz1/Y adult males, to a level that is not significantly different from that seen when a transgene containing wild-type caz is expressed using the same driver.

      Expression of Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 partially rescues the locomotor defects seen in caz1/Y adult males.

      Expression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 does not rescue the locomotor defects seen in caz1/Y adult males.

      Expression of Hsap\FUSScer\UAS.10x.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 completely rescues the reduced longevity of adult caz1/Y escapers.

      Expression of either Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG or Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 does not rescue the the median lifespan of adult caz1/Y escapers, although maximum lifespan is increased.

      (Wang et al., 2011)
      Complementation and Rescue Data
      Rescued by

      caz383 is rescued by Scer\GAL4CCAP.PU/cazUAS.x10.Tag:FLAG

      (Baldwin et al., 2016)

      caz383 is rescued by cazUAS.x10.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Baldwin et al., 2016)

      caz383 is rescued by Scer\GAL4Toll-6-D42/cazP398L.10x.UAS.Tag:FLAG

      (Baldwin et al., 2016)
      Partially rescued by

      caz383 is partially rescued by Scer\GAL4CCAP.PU/cazP398L.10x.UAS.Tag:FLAG

      (Baldwin et al., 2016)
      Comments

      The semi-lethality, the decreased third instar larval crawling capacity and the decreased adult climbing capacity observed in caz383 homozygotes are rescued by the expression of either cazScer\UAS.x10.T:Zzzz\FLAG or cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42.

      The decreased axonal transport of mitochondria in caz383 homozygous third instar larval motor neurons is rescued by the expression of either cazScer\UAS.x10.T:Zzzz\FLAG or cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI; their decreased axonal transport of (NPY-positive) vesicles is rescued by the expression of cazScer\UAS.x10.T:Zzzz\FLAG, but not or cazP398L.10x.Scer\UAS.T:Zzzz\FLAG, under the control of Scer\GAL4CCAP.PI.

      (Baldwin et al., 2016)

      cazT:Zzzz\FLAG rescues the electrophysiological defects seen in 'caz1' mutants (FlyBase curator comment: this mutant corresponds to Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function).

      (Machamer et al., 2014)

      caz+tBID rescues the lethality of males hemizygous for the 'caz1' chromosome (FlyBase curator comment: this chromosome corresponds to Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function).

      (Wang et al., 2012)

      cazT:Zzzz\FLAG fully rescues the reduced adult viability of males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function). The reduced longevity, locomotion defects and external morphological defects of adult caz1/Y escapers are also fully rescued by cazT:Zzzz\FLAG.

      Expression of cazScer\UAS.x10.T:Zzzz\FLAG in the nervous system under the control of Scer\GAL4elav-C155 rescues the adult viability of males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) to 83.7% of control levels. The locomotion defects seen in caz1/Y adult escapers are also strongly, but not completely rescued in these animals, while longevity is completely rescued.

      (Wang et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      caz1
      (Cacciottolo et al., 2019, Baldwin et al., 2016, Machamer et al., 2014, Shahidullah et al., 2013, Wang et al., 2012, Wang et al., 2011)
      caz383
      Name Synonyms
      Secondary FlyBase IDs
        References (6)