Allele Dmel\caz³⁸³

General Information
Symbol	Dmel\caz³⁸³	Species	D. melanogaster
Name		FlyBase ID	FBal0265755
Feature type	allele	Associated gene	Dmel\caz

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Wang et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}caz^EP1564 (Wang et al., 2011)
Nature of the lesion	Statement Reference Imprecise excision of P{EP}caz[EP1564] resulting in a deletion that removes 2443 bp of the caz coding sequence and 3251 bp of the upstream region including the entire caz promoter. The deletion also disrupts CG32576. (Wang et al., 2011)
Caused by aberration	Df(1)383 (Wang et al., 2011)
Carried on aberration	Df(2R)BSC660 (Wang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype, non-enhanceable by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has partially lethal - majority die phenotype, non-enhanceable by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype, non-enhanceable by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011)
Suppressed by
Statement Reference CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype, suppressible \| partially by Scer\GAL4^elav-C155/Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype, suppressible \| partially by Scer\GAL4^elav-C155/Hsap\FUS^{Scer\UAS.10x.T:Zzzz\FLAG} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\FUS^{Scer\UAS.10x.T:Zzzz\FLAG} (Wang et al., 2011)
NOT suppressed by
Statement Reference CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\FUS^{R522G.10x.Scer\UAS.T:Zzzz\FLAG} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype, non-suppressible by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has partially lethal - majority die phenotype, non-suppressible by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\FUS^{R522G.10x.Scer\UAS.T:Zzzz\FLAG} (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011)
Suppressor of
Statement Reference CG32576^Scer\UAS.x10/caz³⁸³, Scer\GAL4^Rapgap1-OK6 is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^Rapgap1-OK6, TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011)
Other
Statement Reference CG32576^Scer\UAS.x10, caz³⁸³/caz^T:Zzzz\FLAG has viable phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has flightless phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has locomotor behavior defective phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has partially lethal - majority die \| P-stage phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has partially lethal - majority die phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has short lived phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz^{Scer\UAS.x10.T:Zzzz\FLAG}/caz³⁸³ has locomotor behavior defective phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155, caz³⁸³ has viable phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, Hsap\FUS^{R522G.10x.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155, caz³⁸³ has viable phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, Hsap\FUS^{Scer\UAS.10x.T:Zzzz\FLAG}, Scer\GAL4^elav-C155, caz³⁸³ has viable phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, Scer\GAL4^elav-C155, caz^{Scer\UAS.x10.T:Zzzz\FLAG}/caz³⁸³ has viable phenotype (Wang et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference CG32576^Scer\UAS.x10/caz³⁸³, Scer\GAL4^Rapgap1-OK6 is a suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Scer\GAL4^Rapgap1-OK6, TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} (Wang et al., 2011)
Other
Statement Reference CG32576^Scer\UAS.x10, caz³⁸³ has eye phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has macrochaeta phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has male genitalia phenotype (Wang et al., 2011) CG32576^Scer\UAS.x10, caz³⁸³ has wing vein phenotype (Wang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Mutants of caz[383] in a CG32576[Scer\UAS.x10] genetic background display defective development. Hemizygous caz[383], CG32576[Scer\UAS.x10]/Y male larvae appear morphologically normal, however, only 14 % of the expected number successfully completed pupariation and eclosed to produce viable adults. caz[T:Zzzz\FLAG] fully rescues the developmental defects seen in hemizygous caz[383], CG32576[Scer\UAS.x10]/Y male larvae. Expression of caz[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] restores viability of caz[383] mutants carrying CG32576[Scer\UAS.x10]. Surviving hemizygous caz[383] mutant adults that also carry CG32576[Scer\UAS.x10] have reduced locomotion and longevity. Externally these mutant males display a mild rough eye defect, abnormal genitalia, and defects in both bristles and wing vein organisation, all of which can be rescued by caz[T:Zzzz\FLAG]. The majority of hemizygous caz[383] mutant males carrying CG32576[Scer\UAS.x10] also exhibit defects in locomotion. The majority of the mutants are unable to fly, and compared with controls, they walk slowly, fall over frequently, and have difficulty righting themselves. All of these phenotypes can be rescued by caz[T:Zzzz\FLAG]. Expression of caz[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in caz[383] mutants also carrying CG32576[Scer\UAS.x10] partially suppresses the locomotion defects in the hemizygous males. However, the partially rescued mutants are still significantly slower than controls. Brains of hemizygous caz[383] mutant males that also carry CG32576[Scer\UAS.x10] do not show signs of extensive neuronal loss, for example, vacuolization. Hemizygous caz[383] mutant males that also carry CG32576[Scer\UAS.x10] have shorter life span than control male flies. caz[T:Zzzz\FLAG] fully restores the average life span of these mutants as does neuronal expression of caz[Scer\UAS.x10.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155]. The neuromuscular junction (NMJ) morphology of hemizygous caz[383] larvae that also carry CG32576[Scer\UAS.x10] appear normal. The NMJ expansion induced by the overexpression of TBPH[Scer\UAS.10x.T:Avic\GFP-YFP.Venus] under the control of Scer\GAL4[Rapgap1-OK6] is completely suppressed in hemizygous caz[383] larvae that also carry CG32576[Scer\UAS.x10]. (Wang et al., 2011)
Xenogenetic Interactions
Statement Reference Expression of Hsap\FUS[Scer\UAS.10x.T:Zzzz\FLAG] in the nervous system of caz[383] mutant larvae (that also carry CG32576[Scer\UAS.x10]) under the control of Scer\GAL4[elav-C155] restores adult viability. Expression of Hsap\FUS[R522G.10x.Scer\UAS.T:Zzzz\FLAG] in the nervous system of caz[383] mutant larvae (that also carry CG32576[Scer\UAS.x10]) under the control of Scer\GAL4[elav-C155] restores adult viability. Expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] in the nervous system of caz[383] mutant larvae (that also carry CG32576[Scer\UAS.x10]) under the control of Scer\GAL4[elav-C155] restores adult viability. Expression of Hsap\FUS[Scer\UAS.10x.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in caz[383] mutants also carrying CG32576[Scer\UAS.x10] partially suppresses the locomotion defects in the hemizygous males. However, the partially rescued mutants are still significantly slower than controls. Expression of Hsap\FUS[R522G.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in hemizygous caz[383] mutant males that also carry CG32576[Scer\UAS.x10] does not suppress the caz[383]-associated locomotory defects. Expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in caz[383] mutants also carrying CG32576[Scer\UAS.x10] partially suppresses the locomotory defects in the hemizygous males. However, the partially rescued mutants are still significantly slower than controls. Neuronal expression of Hsap\FUS[Scer\UAS.10x.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] fully restores the average life span of caz[383] mutants that also carry CG32576[Scer\UAS.x10]. Neuronal expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] does not significantly restore median life span in caz[383] mutants that also carry CG32576[Scer\UAS.x10], although maximum life span is increased. Neuronal expression of Hsap\FUS[R522G.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] does not significantly restore median life span in caz[383] mutants that also carry CG32576[Scer\UAS.x10], although maximum life span is increased. (Wang et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	The recombinant chromosome carrying caz[383] and CG32576[Scer\UAS.x10] is referred to as caz[1] in FBrf0216240. (Wang et al., 2011)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	caz³⁸³
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126 The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240]

Allele Dmel\caz383

Allele Dmel\caz³⁸³