FlyBase Allele Report: Dmel\TBPH[UAS.10x.Venus]

General Information

Symbol

Dmel\TBPH^{UAS.10x.Venus}

Species

D. melanogaster

Name

FlyBase ID

FBal0265761

Feature type

allele

Associated gene

Dmel\TBPH

Associated Insertion(s)

Carried in Construct

P{10xUAS-YFP-TBPH.Venus}

Key Links

Transgenic product class

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

(Wang et al., 2011)

Progenitor genotype

Carried in construct

P{10xUAS-YFP-TBPH.Venus}

Cytology

Description

Ten copies of UAS regulatory sequences drive expression of TBPH with an N-terminal Venus-flag.

(Wang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

TBPH

Tagged with

Venus

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 10

CEC

(Cacciottolo et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by TBPH^Δ23, TBPH¹

(Baldwin et al., 2016)

model of amyotrophic lateral sclerosis type 10

is exacerbated by Gem3^ΔN.UAS

(Cacciottolo et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Toll-6-D42

(Baldwin et al., 2016)

abnormal neuroanatomy, with Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

short lived, with Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

viable, with Scer\GAL4^Toll-6-D42

(Baldwin et al., 2016)

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage, with Scer\GAL4^CCAP.PU

(Baldwin et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

Detailed Description

Statement

Reference

The expression of TBPH^{UAS.10x.Venus} under the control of Scer\GAL4^da.G32 induces significant locomotor defects in adults (progressive decrease in climbing behavior in negative geotaxis assays), but not in larvae.

(Byrne et al., 2017)

The expression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^CCAP.PI leads to a mild decrease in axonal transport of vesicles (i.e. a significant increase in the stationary fraction of NPY-positive vesicles), but not of mitochondria, in third instar larval motor neurons, as compared to controls. Expression under the control of Scer\GAL4^Toll-6-D42 leads to a progressive decrease in adult locomotion (in climbing assays), but has no significant effects in viability or larval locomotion (in crawling assays), as compared to controls.

(Baldwin et al., 2016)

Overexpression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} in motor neurons via Scer\GAL4^RapGAP1-OK6 engenders a dramatic neuromuscular junction (NMJ) expansion.

(Wang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has abnormal locomotor behavior | adult stage | progressive phenotype, non-enhanceable by Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has short lived phenotype, non-enhanceable by Scer\GAL4^nSyb.PS/Ufd1^KK108134

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has abnormal locomotor behavior | adult stage | progressive phenotype, non-enhanceable by Scer\GAL4^nSyb.PS/Ufd1^KK108134

(Byrne et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has short lived phenotype, suppressible | partially by Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^RapGAP1-OK6, TBPH^{UAS.10x.Venus} has abnormal neuroanatomy phenotype, suppressible by CG32576^+t5.0/Df(1)383

(Wang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has short lived phenotype, non-suppressible by Scer\GAL4^nSyb.PS/Ufd1^KK108134

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS, TBPH^{UAS.10x.Venus} has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^nSyb.PS/Ufd1^KK108134

(Byrne et al., 2017)

Enhancer of

Statement

Reference

TBPH^{UAS.10x.Venus}, Scer\GAL4^nSyb.PS is an enhancer of short lived phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS/TBPH^{UAS.10x.Venus} is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^nSyb.PS, Ufd1^KK108134

(Byrne et al., 2017)

NOT Enhancer of

Statement

Reference

TBPH^{UAS.10x.Venus}, Scer\GAL4^nSyb.PS is a non-enhancer of abnormal locomotor behavior | larval stage phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

TBPH^{UAS.10x.Venus}, Scer\GAL4^nSyb.PS is a non-enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS/TBPH^{UAS.10x.Venus} is a non-enhancer of abnormal locomotor behavior | larval stage phenotype of Scer\GAL4^nSyb.PS, Ufd1^KK108134

(Byrne et al., 2017)

NOT Suppressor of

Statement

Reference

TBPH^{UAS.10x.Venus}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

TBPH^{UAS.10x.Venus}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

(Byrne et al., 2017)

Scer\GAL4^nSyb.PS/TBPH^{UAS.10x.Venus} is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Scer\GAL4^nSyb.PS, Ufd1^KK108134

(Byrne et al., 2017)

Scer\GAL4^elav-C155/TBPH^{UAS.10x.Venus} is a non-suppressor of partially lethal - majority die phenotype of CG32576^+t5.0, caz³⁸³

(Wang et al., 2011)

Scer\GAL4^elav-C155/TBPH^{UAS.10x.Venus} is a non-suppressor of short lived phenotype of CG32576^+t5.0, caz³⁸³

(Wang et al., 2011)

Scer\GAL4^elav-C155/TBPH^{UAS.10x.Venus} is a non-suppressor of abnormal locomotor behavior phenotype of CG32576^+t5.0, caz³⁸³

(Wang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, TBPH^{UAS.10x.Venus} has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by CG32576^+t5.0/Df(1)383

(Wang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} in the nervous system under the control of Scer\GAL4^elav-C155 does not rescue the reduced viability, locomotion defects or reduced longevity of adult males hemizygous for the 'caz¹' mutant chromosome (Df(1)383 onto which a transgene containing CG32576^+t5.0 has been recombined to provide CG32576[+] function).

The neuromuscular junction expansion induced by the overexpression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^RapGAP1-OK6 is completely suppressed in a 'caz¹'/Y background.

(Wang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

TBPH^{UAS.10x.Venus} rescues Df(2R)BSC660/TBPH^Δ23

(Wang et al., 2011)

Partially rescues

Scer\GAL4^Toll-6-D42/TBPH^{UAS.10x.Venus} partially rescues TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

Scer\GAL4^CCAP.PU/TBPH^{UAS.10x.Venus} partially rescues TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

Comments

The expression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^Toll-6-D42 rescues the semi-lethality and the decreased third instar larval crawling capacity, but not the adult locomotor capacity, observed in TBPH¹/TBPH^Δ23 transheterozygotes. Scer\GAL4^CCAP.PI-driven expression also partially rescues the decreased axonal transport of mitochondria in TBPH¹/TBPH^Δ23 transheterozygous third instar larval motor neurons.

(Baldwin et al., 2016)

Neuronal expression of TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^elav-C155 fully rescues the locomotory defects, reduced viability and longevity phenotypes of TBPH^Δ23/Df(2R)BSC660 mutants.

(Wang et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

TBPH^{Scer\UAS.10x.T:Avic\GFP-YFP.Venus}

TBPH^{UAS.10x.Venus}

TBPH^WT

(Baldwin et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

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