Allele Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}	Species	H. sapiens
Name		FlyBase ID	FBal0265764
Feature type	allele	Associated gene	Hsap\FUS

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Wang et al., 2011) in vitro construct - coding region fusion (Wang et al., 2011) in vitro construct - regulatory fusion (Wang et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Ten copies of Scer\UAS regulatory sequences drive expression of a P525L mutant Hsap\FUS cDNA fused to an N-terminal T:Zzzz\FLAG epitope tag. (Wang et al., 2011)
Carried in construct	P{10xUAS-FUS.P525L} (Wang et al., 2011)
Tagged with	T:Zzzz\FLAG (Wang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Overexpression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] in motor neurons via Scer\GAL4[Rapgap1-OK6] does not results in neuromuscular junction (NMJ) expansion. Overexpression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] in neurons has no significant effect on locomotion. Overexpression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] has no effect on longevity. (Wang et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^elav-C155/Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG} is a suppressor \| partially of locomotor behavior defective phenotype of CG32576^Scer\UAS.x10, caz³⁸³ (Wang et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG} is a non-suppressor of short lived phenotype of CG32576^Scer\UAS.x10, caz³⁸³ (Wang et al., 2011)
Other
Statement Reference CG32576^Scer\UAS.x10, Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155, caz³⁸³ has viable phenotype (Wang et al., 2011)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] in the nervous system of caz[383] mutant larvae (that also carry CG32576[Scer\UAS.x10]) under the control of Scer\GAL4[elav-C155] restores adult viability. Expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] in caz[383] mutants also carrying CG32576[Scer\UAS.x10] partially suppresses the locomotory defects in the hemizygous males. However, the partially rescued mutants are still significantly slower than controls. Neuronal expression of Hsap\FUS[P525L.10x.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] does not significantly restore median life span in caz[383] mutants that also carry CG32576[Scer\UAS.x10], although maximum life span is increased. (Wang et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wang et al., 2011, J. Clin. Invest. 121(10): 4118--4126 The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span. [FBrf0216240]

Allele Hsap\FUSP525L.10x.Scer\UAS.T:Zzzz\FLAG

Allele Hsap\FUS^{P525L.10x.Scer\UAS.T:Zzzz\FLAG}