Allele Dmel\Lapsyn^Scer\UAS.cGa

General Information
Symbol	Dmel\Lapsyn^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Guan	FlyBase ID	FBal0265768
Feature type	allele	Associated gene	Dmel\Lapsyn

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Guan et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Lapsyn. (Guan et al., 2011)
Carried in construct	P{UAS-Lapsyn} (Guan et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	learning defective \| third instar larval stage, with Lapsyn^zg1, Scer\GAL4^elav-C155 (Guan et al., 2011) lethal \| embryonic stage, with Lapsyn^zg1, Scer\GAL4^Mhc.PU (Guan et al., 2011) lethal \| P-stage, with Lapsyn^zg1, Scer\GAL4^elav-C155 (Guan et al., 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^elav-C155 (Guan et al., 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^how-24B (Guan et al., 2011)
Phenotype Manifest In
	neuromuscular junction \| third instar larval stage, with Scer\GAL4^elav-C155 (Guan et al., 2011) neuromuscular junction \| third instar larval stage, with Scer\GAL4^how-24B (Guan et al., 2011) varicosity \| supernumerary \| third instar larval stage, with Scer\GAL4^elav-C155 (Guan et al., 2011) varicosity \| third instar larval stage, with Scer\GAL4^how-24B (Guan et al., 2011)
Detailed Description
	Statement Reference Presynaptic overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] results in a statistically significant increase in overall varicosity number, without the formation of satellite boutons. Postsynaptic overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[how-24B] results in a statistically significant decrease in synaptic varicosity number. (Guan et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Lapsyn^Scer\UAS.cGa/Scer\GAL4^αTub84B.PL partially rescues Lapsyn^zg1 (Guan et al., 2011) Scer\GAL4^Act.PU/Lapsyn^Scer\UAS.cGa partially rescues Df(2R)Exel6072/Lapsyn^zg1 (Guan et al., 2011) Scer\GAL4^Act.PU/Lapsyn^Scer\UAS.cGa partially rescues Lapsyn^zg1 (Guan et al., 2011) Scer\GAL4^elav-C155/Lapsyn^Scer\UAS.cGa partially rescues [+]/Lapsyn^zg1 (Guan et al., 2011) Scer\GAL4^elav-C155/Lapsyn^Scer\UAS.cGa partially rescues Lapsyn^zg1 (Guan et al., 2011)
Fails to rescue	Lapsyn^Scer\UAS.cGa/Scer\GAL4^Mhc.PU fails to rescue Lapsyn^zg1 (Guan et al., 2011)
Comments	Overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[Act.PU] partially rescues the lethality of both Lapsyn[zg1]/Df(2R)Exel6072 and homozygous Lapsyn[zg1] mutants. The rescued homozygous Lapsyn[zg1] animals emerge as adults and show no obvious behavioural abnormalities. Similar results are obtained using Scer\GAL4[αTub84B.PL]. Overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] partially rescues the lethality of homozygous Lapsyn[zg1]. The rescued animals survive to the pupal stage, but they fail to eclose as adults. Overexpression of Lapsyn[Scer\UAS.cGa] via Scer\GAL4[Mhc.PU] fails to rescue the lethality of homozygous Lapsyn[zg1]. Homozygous Lapsyn[zg1] mutants expressing Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[Mhc.PU] die as embryos. Presynaptic expression of Lapsyn[Scer\UAS.cGa] driven by Scer\GAL4[elav-C155] can rescue the satellite bouton phenotype of heterozygous Lapsyn[zg1]. However, expression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] fails to rescue the learning defects in heterozygous Lapsyn[zg1] larvae. (Guan et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Lapsyn^Scer\UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Guan
Secondary FlyBase IDs

References ( 1 )
Research paper	Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277]

Allele Dmel\LapsynScer\UAS.cGa

Allele Dmel\Lapsyn^Scer\UAS.cGa