|Name||Saccharomyces cerevisiae UAS construct a of Guan||FlyBase ID||FBal0265768|
|Feature type||allele||Associated gene||Dmel\Lapsyn|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Presynaptic overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] results in a statistically significant increase in overall varicosity number, without the formation of satellite boutons. Postsynaptic overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[how-24B] results in a statistically significant decrease in synaptic varicosity number.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Fails to rescue|
Overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[Act.PU] partially rescues the lethality of both Lapsyn[zg1]/Df(2R)Exel6072 and homozygous Lapsyn[zg1] mutants. The rescued homozygous Lapsyn[zg1] animals emerge as adults and show no obvious behavioural abnormalities. Similar results are obtained using Scer\GAL4[αTub84B.PL]. Overexpression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] partially rescues the lethality of homozygous Lapsyn[zg1]. The rescued animals survive to the pupal stage, but they fail to eclose as adults. Overexpression of Lapsyn[Scer\UAS.cGa] via Scer\GAL4[Mhc.PU] fails to rescue the lethality of homozygous Lapsyn[zg1]. Homozygous Lapsyn[zg1] mutants expressing Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[Mhc.PU] die as embryos. Presynaptic expression of Lapsyn[Scer\UAS.cGa] driven by Scer\GAL4[elav-C155] can rescue the satellite bouton phenotype of heterozygous Lapsyn[zg1]. However, expression of Lapsyn[Scer\UAS.cGa] under the control of Scer\GAL4[elav-C155] fails to rescue the learning defects in heterozygous Lapsyn[zg1] larvae.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Guan
|Secondary FlyBase IDs|
|References ( 1 )|